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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724397.2
Proper citation: RRID:Addgene_152527 Copy
Species: Homo sapiens
Genetic Insert: v-ral simian leukemia viral oncogene homolog A
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17540176
Proper citation: RRID:Addgene_15251 Copy
Genetic Insert: hChR2(H134R)-P2A-EYFP
Vector Backbone Description: Vector Backbone:pAAV-EF1a-DIO; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32073399
Proper citation: RRID:Addgene_139283 Copy
Species: Mus musculus
Genetic Insert: Wnt-4
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Mouse Wnt-4 full-length ORF:
5’BsaI-primer: GGTCTCCATG AGCCCCCGTT CGTGC
3’EcoRI-primer: CTGAATTCAC CGGCACGTGT GCATCTCC.
Proper citation: RRID:Addgene_13937 Copy
Genetic Insert: linker with pelB-leader, cloning site, flag-tag and p3
Vector Backbone Description: Vector Backbone:fADL-1e; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32872428
Proper citation: RRID:Addgene_139441 Copy
Species: Synthetic
Genetic Insert: no sgRNA inserted; resistance gene: neoR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Comments: Generated by replacing PuroR gene with NeoR gene in LentiGuide-Puro from Feng Zhang (Addgene plasmid # 52963).
Proper citation: RRID:Addgene_139449 Copy
Species: Homo sapiens
Genetic Insert: FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform)
Vector Backbone Description: Backbone Marker:PMID: 24048477; Vector Backbone:RRL.sin.cPPT.CMV/IRES-puro.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139447 Copy
Species: Synthetic
Genetic Insert: Non-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139456 Copy
Genetic Insert: DTECT ligation product
Vector Backbone Description: Backbone Size:3500; Vector Backbone:TOPO; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139333 Copy
Species: Synthetic
Genetic Insert: Non-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139455 Copy
Species: Synthetic
Genetic Insert: Curlcake4 with T7 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501426
Proper citation: RRID:Addgene_139343 Copy
Species: Synthetic
Genetic Insert: Curlcake2 with T7 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501426
Proper citation: RRID:Addgene_139341 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with 267nt of 5' UTR
Vector Backbone Description: Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961910
Proper citation: RRID:Addgene_139344 Copy
Species: Other
Genetic Insert: membrane-EGFP
Vector Backbone Description: Backbone Size:3203; Vector Backbone:pRN3P; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31118510
Proper citation: RRID:Addgene_139402 Copy
Species: Synthetic, Aequorea victoria, Streptomyces noursei
Genetic Insert: GFP S65T
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32145317
Comments: Contains the non-codon optimised GFP S65T marker in the multicloning site of pBlueScript II SK-. Importantly, the GFP S65T marker can be excised using restriction enzymes XhoI and SpeI.
Proper citation: RRID:Addgene_139410 Copy
Species: Gallus gallus
Genetic Insert: Wnt-14
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11239392
Comments: Full-length ORF of chick Wnt14.
Proper citation: RRID:Addgene_13948 Copy
Species: Mus musculus
Genetic Insert: Pax2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11804780
Comments: The Tabin lab only used this clone for in situ hybridization. Pax2 activity was never tested.
Proper citation: RRID:Addgene_13960 Copy
Species: Drosophila melanogaster
Genetic Insert: Adenosine deaminase acting on RNA
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29127211
Proper citation: RRID:Addgene_139686 Copy
Genetic Insert: shSCR
Vector Backbone Description: Backbone Size:7538; Vector Backbone:pLKO.1C-shSCR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32141762
Comments: pLKO.1 : https://www.addgene.org/10878/
CMV-HIV hybrid promoter : Amendola M, Venneri MA, Biffi A, Vigna E, Naldini L. Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters. Nat. Biotechnol. 23 (1), 108-116 (2005).
Proper citation: RRID:Addgene_139647 Copy
Species: S. pyogenes
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:8828; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139651 Copy
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