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Genetic Insert: G-GECO1.2
Vector Backbone Description: Backbone Size:4200; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903779
Comments: The vector is modified from pBAD/ His B (Invitrogen).
Proper citation: RRID:Addgene_32468 Copy
Species: synthetic construct
Genetic Insert: R-GECO1.0
Vector Backbone Description: Backbone Size:4200; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903779
Comments: The vector is modified from pBAD/ His B (Invitrogen).
Proper citation: RRID:Addgene_32465 Copy
Species: Mus musculus
Genetic Insert: CAMKIIalpha promoter 1.3kb version
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32577 Copy
Species: Gallus gallus
Genetic Insert: 1XDR4
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32614 Copy
Species: Gallus gallus
Genetic Insert: Thyroid Response Element
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32612 Copy
Genetic Insert: FLPo
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32650 Copy
Species: Mus musculus
Genetic Insert: Kir2.1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157418
Comments: Addgene's sequencing results identified a single nucleotide insertion at bp#60/61 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.
Proper citation: RRID:Addgene_32641 Copy
Species: Mus musculus
Genetic Insert: tetO-Kir2.1-IRES-TLZ
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157418
Comments: Addgene's sequencing results identified a single nucleotide a single nucleotide deletion at bp#1135 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.
Proper citation: RRID:Addgene_32642 Copy
Genetic Insert: ChR2
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32646 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32649 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: The genes which replaced GFP in the pcDNA-SADdeltaG-GFP plasmid were amplified by
PCR using plasmids encoding mCherry and primer pairs with BamHI and NheI/NotI
sequences at the ends.
Proper citation: RRID:Addgene_32636 Copy
Species: Rabies virus
Genetic Insert: B19N, B19P, B19M and B19L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5015; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: To construct the rabies virus genomic cDNA, several pieces of rabies genomic cDNA were ligated with unique restriction enzyme sites, AgeI, MluI, BlpI, BstZ17I, and BstBI. Addition of HamRz and HdvRz sequences were performed by PCR using synthesized primers designed to attach these ribozyme sequences.
The pSADdeltaG-F3 has 3 unique restriction enzyme sites Sbf1, Srf1 and NheI for cloning the first gene and 3 unique restriction enzyme sites PacI, AscI and SacII for cloning the second gene between B19M and B19L.
Proper citation: RRID:Addgene_32634 Copy
Genetic Insert: Sce
Vector Backbone Description: Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_32628 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: oChiEF
Vector Backbone Description: Backbone Marker:Addgene plasmid 20071; Backbone Size:4885; Vector Backbone:pACAGW-ChR2-Venus-AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23029198
Comments: Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.
Proper citation: RRID:Addgene_32846 Copy
Species: Homo sapiens
Genetic Insert: OCLN
Vector Backbone Description: Backbone Marker:Gungxia Gao; Vector Backbone:pTrip; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20822789
Proper citation: RRID:Addgene_32794 Copy
Species: Homo sapiens
Genetic Insert: AMOT p80
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Proper citation: RRID:Addgene_32830 Copy
Species: Homo sapiens
Genetic Insert: AMOT p130
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Comments: E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.
Proper citation: RRID:Addgene_32829 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138822
Proper citation: RRID:Addgene_32703 Copy
Species: Homo sapiens
Genetic Insert: AMOT p130
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Comments: E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.
Proper citation: RRID:Addgene_32828 Copy
Genetic Insert: emGFP-miRNA
Vector Backbone Description: Backbone Marker:Richard O. Hynes group; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138822
Proper citation: RRID:Addgene_32704 Copy
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