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Species: Synthetic
Genetic Insert: Levoglucosan kinase wild-type
Vector Backbone Description: Backbone Size:6109; Vector Backbone:pETconNK; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28196882
Proper citation: RRID:Addgene_81165 Copy
Species: Synthetic
Genetic Insert: Retro-BCMACAR
Vector Backbone Description: Vector Backbone:pRetro; Vector Types:Retroviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35276062
Proper citation: RRID:Addgene_192199 Copy
Species: Synthetic
Genetic Insert: Retro-HER2CAR
Vector Backbone Description: Vector Backbone:pRetro; Vector Types:Retroviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35276062
Proper citation: RRID:Addgene_192201 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810) ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36366748
Comments: 3rd generation lentiviral backbone.
Please visit https://doi.org/10.1101/2022.02.28.482127 for bioRxiv preprint.
Proper citation: RRID:Addgene_189947 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810) ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36366748
Comments: 3rd generation lentiviral backbone.
Please visit https://doi.org/10.1101/2022.02.28.482127 for bioRxiv preprint.
Proper citation: RRID:Addgene_189951 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810) ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36366748
Comments: 3rd generation lentiviral backbone.
Please visit https://doi.org/10.1101/2022.02.28.482127 for bioRxiv preprint.
Proper citation: RRID:Addgene_189952 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pMGU; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139933 Copy
Species: Synthetic
Genetic Insert: LacI-Ptac-sfGFP
Vector Backbone Description: Vector Backbone:pMGA; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139934 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pMGA; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31922737
Proper citation: RRID:Addgene_139931 Copy
Species: Synthetic
Genetic Insert: TetR-Ptet-sfGFP
Vector Backbone Description: Vector Backbone:pMGA; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139935 Copy
Species: Synthetic
Genetic Insert: BE3
Vector Backbone Description: Backbone Marker:Mumberg et al. (Nucleic Acids Research, 1994, Vol. 22, No. 25); Backbone Size:6653; Vector Backbone:p415-GALL; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36326816
Proper citation: RRID:Addgene_172409 Copy
Species: Synthetic
Genetic Insert: mPlum
Vector Backbone Description: Vector Backbone:pMax; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24757057
Comments: For more information, see Piett, Pecen et al. 2021 Nat Protoc. (doi: 10.1038/s41596-021-00577-3).
This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but it may reduce transformation efficiencies. If you need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_177830 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185665 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185662 Copy
Species: Synthetic
Genetic Insert: Ezr sgRNA
Vector Backbone Description: Backbone Marker:Khakh lab; Backbone Size:5206; Vector Backbone:PZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378959
Proper citation: RRID:Addgene_179119 Copy
Species: Synthetic
Genetic Insert: Fermt2 sgRNA
Vector Backbone Description: Backbone Marker:Khakh lab; Backbone Size:5206; Vector Backbone:PZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378959
Proper citation: RRID:Addgene_179117 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185666 Copy
Species: Synthetic
Genetic Insert: gRNA cassette
Vector Backbone Description: Backbone Marker:Mumberg et al. (Nucleic Acids Research, 1994, Vol. 22, No. 25); Backbone Size:6145; Vector Backbone:p426; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36326816
Proper citation: RRID:Addgene_172517 Copy
Species: Synthetic
Genetic Insert: FOLD6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: Backbone is identical to that of Addgene #37129 (several amino acids between the FP insert and His-tag are removed).
Proper citation: RRID:Addgene_186547 Copy
Species: Synthetic
Genetic Insert: LSSA12
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36344833
Comments: 398 nm excitation, 511 nm emission. Backbone is identical to that of Addgene #37129 (several amino acids between the FP insert and His-tag are removed).
Proper citation: RRID:Addgene_186546 Copy
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