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Species: HIV-I
Genetic Insert: NL43-dGPE-EGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Comments: This plasmid is based on pfNL43-dE-EGFP.
Addgene's sequencing results identified 4 nucleotide mismatches at bp# 1634 & 2223-2225 when compared to the full plasmid sequence provided by the depositing laboratory, are remnants of the deleted pol gene and are not a concern for the function of the plasmid. According to the depositing laboratory, this plasmid still produces infectious virus when gag, pol and env are supplied in trans.
Proper citation: RRID:Addgene_36866 Copy
Species: Danio rerio
Genetic Insert: Hox B7a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe Synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9425136
Comments: Please note that Addgene's sequencing results indicate the Hox B7a insert in this plasmid starts at amino acid 143 when compared to GenBank entry NP_001108563.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
Proper citation: RRID:Addgene_36861 Copy
Species: Danio rerio
Genetic Insert: Hox B8a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe Synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9425136
Comments: Please note that Addgene's sequencing results indicate the Hox B8a insert in this plasmid has a D238E mutation when compared to GenBank entry NP_571195.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
Proper citation: RRID:Addgene_36862 Copy
Species: Homo sapiens
Genetic Insert: PBGD fragment
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Comments: PBGD fragment is located at bp# 294-366
Proper citation: RRID:Addgene_36869 Copy
Species: Homo sapiens
Genetic Insert: CCND1-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGGGGTACGCGCGGCGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36854 Copy
Species: Homo sapiens
Genetic Insert: SIRT6-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCGGGAGGCCGCACTTG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36844 Copy
Species: Homo sapiens
Genetic Insert: WTX T289A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1 (with Venus added); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36965 Copy
Species: Homo sapiens
Genetic Insert: VHL-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCTGGATCGCGGAGGGAA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36845 Copy
Species: Homo sapiens
Genetic Insert: RUNX1-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TTCAGGAGGAAGCGATGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36841 Copy
Species: Homo sapiens
Genetic Insert: XPA-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TAAAGCCGCCGCCTCCGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36848 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12972608
Comments: Sugden lab plasmid p3051
Proper citation: RRID:Addgene_36953 Copy
Genetic Insert: LMP1
Vector Backbone Description: Backbone Marker:Addgene plasmid #36953; Backbone Size:7037; Vector Backbone:pCMMP-MCS-IRES-eGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3455
Please note that the full plasmid sequence provided by the depositing laboratory and Addgene's sequencing results differ from GenBank entry YP_401722.1 by three polymorphisms: F106Y, I122L and M129I. These differences result from the particular EBV isolate. For more information please see the following article: https://www.ncbi.nlm.nih.gov/pubmed/7931160
Proper citation: RRID:Addgene_36955 Copy
Species: Homo sapiens
Genetic Insert: WTX
Vector Backbone Description: Backbone Marker:Moon Lab Plasmid available from Addgene (plasmid #15100); Backbone Size:5500; Vector Backbone:pGLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17510365
Comments: Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .
Proper citation: RRID:Addgene_36956 Copy
Species: Homo sapiens
Genetic Insert: EglN2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6886; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36950 Copy
Species: Homo sapiens
Genetic Insert: EglN3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36951 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18042799
Comments: Sugden lab plasmid p3048
Proper citation: RRID:Addgene_36952 Copy
Species: Homo sapiens
Genetic Insert: Thioredoxin 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22430737
Proper citation: RRID:Addgene_36944 Copy
Species: Homo sapiens
Genetic Insert: Thioredoxin-1 C35S mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22430737
Proper citation: RRID:Addgene_36947 Copy
Species: Aequorea victoria
Genetic Insert: Venus (EYFP)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the laboratory of an HHMI investigator
Proper citation: RRID:Addgene_36948 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COF1-RFP
Vector Backbone Description: Vector Backbone:pRS416-URA,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332110
Proper citation: RRID:Addgene_37103 Copy
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