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Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Note that Addgene NGS identified a nonsense mutation in the CcaS insert
Proper citation: RRID:Addgene_50548 Copy
Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
Proper citation: RRID:Addgene_50552 Copy
Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Proper citation: RRID:Addgene_50551 Copy
Species: Drosophila melanogaster
Genetic Insert: Argonaute2
Vector Backbone Description: Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAFW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20605501
Comments: Please note that when compared to GenBank reference sequence NP_648775.1, the Ago2 sequence in this plasmid is missing amino acids 49-54 (LQQPQQ).
Proper citation: RRID:Addgene_50554 Copy
Species: Synthetic
Genetic Insert: Luciferase-let7-Poly(A)
Vector Backbone Description: Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAHW; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23123195
Comments: This plasmid expresses a Renilla luciferase reporter for let-7 miRNA with a regular polyadenylation signal.
Proper citation: RRID:Addgene_50556 Copy
Species: Renilla, unknown
Genetic Insert: Luciferase-A114-N40
Vector Backbone Description: Backbone Marker:GeneScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22117217
Comments: Plasmid contains a defined A114 sequence followed by an EcoT22I site and a 40-bp arbitrary sequence downstream of Renilla lucferase.
Unstable when transformed and so any retransformation must be checked by digest or sequencing before use. In strain MV1184, it is stable as a glycerol stock. Please note that the Rluc coding sequence contains an EcoT22I site. To prepare the T7 transcription template for A114 RNA (without N40), the plasmid should be first linearized by NdeI upstream of the T7 promoter sequence, followed by a partial digestion by EcoT22I. The DNA fragment terminating immediately downstream of the A114 sequence should then be gel purified. For A114-N40 RNA, the plasmid should be digested by HindIII downstream of the N40 sequence.
Proper citation: RRID:Addgene_50561 Copy
Genetic Insert: Limonene Synthase
Vector Backbone Description: Vector Backbone:E. coli DH10B; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727191
Proper citation: RRID:Addgene_50603 Copy
Species: Other
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24287550
Proper citation: RRID:Addgene_50685 Copy
Genetic Insert: tyrB
Vector Backbone Description: Vector Backbone:pRBS-01; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22020510
Proper citation: RRID:Addgene_50606 Copy
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:7363; Vector Backbone:pTAL4; Vector Types:Yeast Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124327
Comments: Addgene's quality control sequencing has found a few mismatches with the depositor's full sequence, but these discrepancies are not thought to affect plasmid function.
Proper citation: RRID:Addgene_50608 Copy
Species: Synthetic
Genetic Insert: DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRS426 Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11000112
Comments: There is a H to T mutation in front of the MetBetaGal. The mutation should not affect function.
Proper citation: RRID:Addgene_50572 Copy
Species: Mus musculus
Genetic Insert: UBR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50573 Copy
Species: Xanthamonas
Genetic Insert: NS3
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50611 Copy
Species: Xanthamonas
Genetic Insert: NS9
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50617 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50582 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50580 Copy
Vector Backbone Description: Vector Backbone:pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50624 Copy
Vector Backbone Description: Vector Backbone:pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers; Vector Types:Mammalian Expression, Lentiviral, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50626 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50587 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50589 Copy
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