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Species: Mus musculus
Genetic Insert: VCP R638A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12368 Copy
Species: Mus musculus
Genetic Insert: VCP D598N
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12365 Copy
Species: Mus musculus
Genetic Insert: VCP D592N
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12364 Copy
Species: Mus musculus
Genetic Insert: VCP R599K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12367 Copy
Species: Mus musculus
Genetic Insert: VCP R586K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12363 Copy
Species: Mus musculus
Genetic Insert: VCP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12373 Copy
Species: Mus musculus
Genetic Insert: mSOD2 shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:UbC-TRE-tTS-IRES-EGFP; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16869515
Comments: See Additional Documents link above for cloning protocol, map, and annotated sequence from the author.
Note that there is a EcoRI and a KpnI site in the polylinker region. You will need to cut the plasmid with BamHI and religate to eliminate these sites before you could EcoRI and KpnI for cloning.
Proper citation: RRID:Addgene_12390 Copy
Species: Mus musculus
Genetic Insert: transferrin receptor
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The murine TfR sequence bp 367-2292 (of the ORF) was cloned into the pAcGP67A vector. The construct includes a short 3' UTR (past the stop codon at bp 2292). The TfR fragment was PCR amplified to introduce an EcoRI site at the 5' end and an EagI site at the 3' end. This was subsequently cloned into the corresponding EcoRI/EagI sites of pAcGP67A. The murine TfR was fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site.
Proper citation: RRID:Addgene_12392 Copy
Species: Mus musculus
Genetic Insert: mSOD2 shRNA
Vector Backbone Description: Backbone Size:6500; Vector Backbone:UbC-EGFP; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16869515
Comments: See Additional Documents link above for cloning protocol, map, and annotated sequence from the author.
Proper citation: RRID:Addgene_12391 Copy
Species: Mus musculus
Genetic Insert: IkB alpha K->A
Vector Backbone Description: Backbone Size:11500; Vector Backbone:RCAS; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12408 Copy
Species: Mus musculus
Genetic Insert: IkB alpha M
Vector Backbone Description: Backbone Size:11500; Vector Backbone:RCAS; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12407 Copy
Species: Mus musculus
Genetic Insert: TSP4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescriptII KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12415 Copy
Species: Mus musculus
Genetic Insert: IRES-Pac, Pou5f1 enhancer
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124181 Copy
Species: Mus musculus
Genetic Insert: FLAG-Esrrb-IRES-H2B-mKO2
Vector Backbone Description: Vector Backbone:pPB-TREtight-cHA-IRESH2BmKO2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124178 Copy
Species: Mus musculus
Genetic Insert: Esrrb-FLAG-IRES-H2B-mKO2
Vector Backbone Description: Vector Backbone:pPB-TREtight-cHA-IRESH2BmKO2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124179 Copy
Species: Mus musculus
Genetic Insert: metaxin 2
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pGBT9.C; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12419 Copy
Species: Mus musculus
Genetic Insert: BDNF
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4600; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661931
Proper citation: RRID:Addgene_124279 Copy
Species: Mus musculus
Genetic Insert: GLI-Kruppel family member GLI3
Vector Backbone Description: Backbone Marker:Thermo Scientific (CloneJET PCR Cloning Kit); Backbone Size:2974; Vector Backbone:pJET1.2/blunt; Vector Types:In situ probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31169497
Proper citation: RRID:Addgene_124440 Copy
Species: Mus musculus
Genetic Insert: RAB28, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific (CloneJET PCR Cloning Kit); Backbone Size:2974; Vector Backbone:pJET1.2/blunt; Vector Types:In situ probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31169497
Proper citation: RRID:Addgene_124442 Copy
Species: Mus musculus
Genetic Insert: GLI-Kruppel family member GLI3
Vector Backbone Description: Backbone Marker:Thermo Scientific (CloneJET PCR Cloning Kit); Backbone Size:2974; Vector Backbone:pJET1.2/blunt; Vector Types:In situ probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31169497
Proper citation: RRID:Addgene_124439 Copy
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