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Species: Synthetic
Genetic Insert: P2A-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27358232
Proper citation: RRID:Addgene_84329 Copy
Species: Homo sapiens
Genetic Insert: GEFT
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmVenus-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27833100
Proper citation: RRID:Addgene_84340 Copy
Species: Streptococcus pyogenes
Genetic Insert: dcas9
Vector Backbone Description: Backbone Marker:Addgene 20315; Backbone Size:4640; Vector Backbone:pTC-0X-1L; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27407107
Proper citation: RRID:Addgene_84379 Copy
Species: Mus musculus
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAd-Track-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15744310
Proper citation: RRID:Addgene_8440 Copy
Species: Homo sapiens
Genetic Insert: PRDM14
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5422; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27281218
Proper citation: RRID:Addgene_84368 Copy
Species: Mus musculus
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAd-Track-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15744310
Proper citation: RRID:Addgene_8439 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:3034; Vector Backbone:pMuLE ENTR MCS L1-R5; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30061623
Comments: This truncated CMV promoter is not silenced in neurons.
Proper citation: RRID:Addgene_84391 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:3028; Vector Backbone:pMuLE ENTR MCS L1-R5; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: This truncated CMV promoter is not silenced in neurons.
Proper citation: RRID:Addgene_84392 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:3076; Vector Backbone:pMuLE ENTR MCS L1-R5; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: This truncated CMV promoter is not silenced in neurons.
Proper citation: RRID:Addgene_84388 Copy
Genetic Insert: EPN01(∆L1+∆L2)-His
Vector Backbone Description: Backbone Size:5214; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_84511 Copy
Genetic Insert: EPN01(∆M)-His
Vector Backbone Description: Backbone Size:5214; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_84510 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUMVC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12649500
Comments: Packaging plasmid for MuLV constructs, MuLV gag and pol (no envelope). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html
See pUMVC3-gag-pol for a map/sequence.
Proper citation: RRID:Addgene_8449 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway donor plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25800674
Comments: No stop codon follows insert.
Proper citation: RRID:Addgene_84525 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway donor plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25800674
Comments: Mutation numbering may differ from listed mutations, but depositor confirms that the correct mutations are present within the plasmid.
Proper citation: RRID:Addgene_84527 Copy
Vector Backbone Description: Vector Backbone:pMycoFos; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21689690
Comments: For maintenance of vector, any standard E.coli is OK, but to make plasmid preps, the plasmid must be propagated in EPI300 or similar strain that enables the arabinose-inducible copy control system. Induction of gene expression is with acetamide in Mycobacterium, but this doesnt work in E.coli. Some level of control over expression in E.coli can be gained via controlling the copy number of the plasmid with arabinose. See published paper for more details on construction and use of pMycoFos. Has unique EcoRI, PacI, NheI, SwaI, BamHI sites for cloning
Proper citation: RRID:Addgene_84577 Copy
Vector Backbone Description: Vector Backbone:pUS232; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: A derivative of pBBR1MCS-2. Non-mobilisable. Cloning site is compatible with iGEM cloning protocols (EcoRI-NotI-XbaI-SpeI-NotI-PstI) and Golden-Gate cloning protocols (BsaI-BsaI). Blue/white selection is via excision of lacZalpha fragment, rather than interruption of this. Has unique ScaI, BamHI, SmaI, EcoRV, EcoRI, XbaI, StuI, SpeI, PstI, HindIII, ClaI, SalI sites for cloning. Has two NotI and BsaI sites for cloning
Proper citation: RRID:Addgene_84579 Copy
Vector Backbone Description: Vector Backbone:pUS116; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: pUS116 is an E.coli/Mycobacterium shuttle vector, with high copy number in E.coli and low copy number in Mycobacterium. The plasmid has unique NdeI, BamHI, PvuII, EcoRI, NheI sites for cloning, and genes cloned at this MCS can be expressed via acetamide induction in Mycobacterium, but this system doesnt work in E.coli. This plasmid was made by deletion of the recombineering genes from pJV53 (see van Kessel and Hatfull, 2007), to re-forma basic shuttle/expression vector. pUS116 is equivalent to vector pLAM12.
Proper citation: RRID:Addgene_84578 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway donor plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25800674
Comments: Stop codon follows rap1 CDS.
Proper citation: RRID:Addgene_84565 Copy
Species: Mus musculus
Genetic Insert: Srap1
Vector Backbone Description: Backbone Marker:Millipore; Backbone Size:5400; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29020633
Proper citation: RRID:Addgene_84568 Copy
Species: Arabidopsis thaliana
Genetic Insert: sAPX
Vector Backbone Description: Backbone Marker:pGWB2; Backbone Size:16636; Vector Backbone:pGemini (Dr. Andrew Simkin); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84735 Copy
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