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Species: Other
Genetic Insert: ecTadA(8e)-nSpCas9 (CP1041)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138493 Copy
Species: Other
Genetic Insert: ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138497 Copy
Species: Other
Genetic Insert: L. monocytogenes PI-PLC(188-287):IGTAGPRSANS[GA]4: MTOR(2021-2113):iRFP
Vector Backbone Description: Vector Backbone:piRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139306 Copy
Species: Other
Genetic Insert: TagBFP2:FKBP1A(3-108):[GGSA]4GG:L. monocytogenes PI-PLC(1-187)
Vector Backbone Description: Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139307 Copy
Species: Other
Genetic Insert: LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
Vector Backbone Description: Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139308 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage PP7
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of PP7CP (amplified from pPP7_mCherry, ID: 61763) was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140262 Copy
Species: Other
Genetic Insert: L7Ae
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of L7Ae was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140263 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:doi:10.1038/nchembio.1366; Backbone Size:14245; Vector Backbone:pKW20088; Vector Types:CRISPR, Synthetic Biology, Proof-of-concept test target of a in filamentous fungi.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32526136
Comments: The PelcA-mCherry has no observable basal expression in Aspergillus nidulans, constituting a good test target for CRISPRa. Also contains PacI NotI sites to clone other components, and allows cloning in Saccharomyces cerevisiae. The mCherry mutation G174D does not appear to affect fluorescence.
Proper citation: RRID:Addgene_140202 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pGbait; Vector Types:Mouse Targeting; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140283 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:doi:10.1038/nchembio.1366; Backbone Size:14245; Vector Backbone:pKW20088; Vector Types:CRISPR, Synthetic Biology, Proof-of-concept test target of a in filamentous fungi.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32526136
Comments: To be cotransformed in strains expressing dSpCas9-VPR. This proof-of-concept vector is intended to serve as a positive control and aid the implementation of the CRISPRa vector set in other fungal species, facilitating initial testing and troubleshooting. The proof of concept activation is easily observable in Aspergillus nidulans with fluorescence microscopy when Cas9 is expressed from pCRI006. The mCherry mutation G174D does not appear to affect fluorescence.
Proper citation: RRID:Addgene_140205 Copy
Species: Other
Genetic Insert: sgRNA for pAID-EF1a plasmid digestion
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32941625
Comments: pX330 plasmid was obtained from Addgene (#42230).
Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140609 Copy
Species: Other
Genetic Insert: 4xHRE-MinTK-CRE-ODD
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141147 Copy
Species: Other
Genetic Insert: MCS and YFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145274 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:Addgene Plasmid #64323; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076284
Proper citation: RRID:Addgene_170182 Copy
Species: Other
Genetic Insert: AsCas12f1 and sgRNA_v1
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34475565
Proper citation: RRID:Addgene_171614 Copy
Species: Other
Genetic Insert: IRES
Vector Backbone Description: Backbone Marker:Michael Davidson; Backbone Size:5395; Vector Backbone:pClover-mRuby2-FRET-10; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33963178
Proper citation: RRID:Addgene_171059 Copy
Species: Other
Genetic Insert: OsTIR1-F74A
Vector Backbone Description: Vector Backbone:pDB4697; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34849776
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171127 Copy
Species: Other
Genetic Insert: nuclear-localized EGFP + FlpO
Vector Backbone Description: Backbone Marker:Matthias J. Schnell, Thomas Jefferson University; Backbone Size:14070; Vector Backbone:Rabies CVS N2c(deltaG); Vector Types:Neurotropic virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36040301
Comments: Please visit https://doi.org/10.1101/2021.12.23.474014 for bioRxiv preprint.
Proper citation: RRID:Addgene_172379 Copy
Species: Other
Genetic Insert: tdTomato + FlpO
Vector Backbone Description: Backbone Marker:Matthias J. Schnell, Thomas Jefferson University; Backbone Size:14070; Vector Backbone:Rabies CVS N2c(deltaG); Vector Types:Neurotropic virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36040301
Comments: Please visit https://doi.org/10.1101/2021.12.23.474014 for bioRxiv preprint.
Proper citation: RRID:Addgene_172375 Copy
Species: Other
Genetic Insert: EGFP + ChIEF
Vector Backbone Description: Backbone Marker:Matthias J. Schnell, Thomas Jefferson University; Backbone Size:14070; Vector Backbone:Rabies CVS N2c(deltaG); Vector Types:Neurotropic virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36040301
Comments: Please visit https://doi.org/10.1101/2021.12.23.474014 for bioRxiv preprint.
Proper citation: RRID:Addgene_172371 Copy
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