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Species: Mus musculus
Genetic Insert: Tgfb3
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32352930
Proper citation: RRID:Addgene_164429 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164452 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_453m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164453 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-mscRE4-minCMV-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
The Allen Institute for Brain Science recommends this plasmid as a potential substitute for Plasmid 163471: AiP1269-scAAV-mscRE4-minBGpromoter-SYFP2-WPRE3-bGHpA.
Proper citation: RRID:Addgene_164457 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_460m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33789083
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164455 Copy
Genetic Insert: VSV-G
Vector Backbone Description: Vector Backbone:HDM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33412089
Proper citation: RRID:Addgene_164440 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34688656
Proper citation: RRID:Addgene_164445 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-868, P801G)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34688656
Proper citation: RRID:Addgene_164444 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34688656
Proper citation: RRID:Addgene_164447 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164599 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164597 Copy
Species: Synthetic
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8037; Vector Backbone:pLVX-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31346185
Proper citation: RRID:Addgene_164590 Copy
Species: Mus musculus
Genetic Insert: (Cyto)iGlucoSnFR-mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161775
Comments: The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (https://www.addgene.org/244084/) instead of this plasmid.
Please visit https://doi.org/10.1101/571422 for bioRxiv preprint.
Proper citation: RRID:Addgene_164514 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pRP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon-optimized SARS-CoV-2 Spike was cloned from SinoBiological plasmid VG40589-UT. An 18-amino acid truncation was introduced to improve expression. The plasmid can be used to generate Spike-pseudotyped lentiviral or VSV particles.
Proper citation: RRID:Addgene_164583 Copy
Species: B. subtilis
Genetic Insert: TEV-SacB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6500; Vector Backbone:pET32a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Designed and verified by Md Rezaul Karim (PhD), the responsible scientist. This plasmid is designed to clone a target gene replacing the sacB gene using CPEC (LIC) cloning. Growth media or agar plate with sucrose works as a negative selection marker for the intact template plasmid with the sacB gene and a positive selection marker for the successfully cloned plasmid.
Proper citation: RRID:Addgene_164588 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8071; Vector Backbone:pLVX-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31346185
Proper citation: RRID:Addgene_164589 Copy
Species: Drosophila melanogaster
Genetic Insert: U6 and DR
Vector Backbone Description: Backbone Size:7350; Vector Backbone:white+attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616113
Proper citation: RRID:Addgene_164586 Copy
Species: Mus musculus
Genetic Insert: Ubiquitin noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164530 Copy
Species: Mus musculus
Genetic Insert: Smoothened noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164533 Copy
Species: Mus musculus
Genetic Insert: Smoothened noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164534 Copy
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