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Species: peach (Prunus persica)
Genetic Insert: eIFiso4E
Vector Backbone Description: Backbone Marker:Dr. Rao (University of California at Riverside); Backbone Size:11000; Vector Backbone:pCass4Rz; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27565765
Proper citation: RRID:Addgene_86092 Copy
Species: Arabidopsis thaliana
Genetic Insert: FLS2
Vector Backbone Description: Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16510871
Proper citation: RRID:Addgene_86158 Copy
Species: Arabidopsis thaliana
Genetic Insert: FLS2
Vector Backbone Description: Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16510871
Proper citation: RRID:Addgene_86157 Copy
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_86176 Copy
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_86177 Copy
Species: Saccharopolyspora erythraea, Strain NRRL 2338
Genetic Insert: Ribonuclease inhibitor StI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5252; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14741361
Comments: Production plasmid for ribonuclease inhibitor and barstar
homolog StI from Saccharopolyspora erythraea. Structural gene for inhibitor StI from Saccharopolyspora
erythraea strain NRRL 2338. N-terminal serine of wild type
changed to glycine and 19 silent mutations improve production
in E. coli.
Proper citation: RRID:Addgene_8616 Copy
Species: Homo sapiens
Genetic Insert: ZNF644 homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_ZNF644_1 and PX458_ZNF644_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF644. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86264 Copy
Species: Homo sapiens
Genetic Insert: KLF11 homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_KLF11_1 and PX458_KLF11_2 into HepG2 cells, and genomic editing was verified by PCR. The donor nucleotide sequence in the final codon of the KLF11 gene is modified to discourage sgRNA recognition, while retaining the native amino acid sequence. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified KLF11. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86269 Copy
Species: Homo sapiens
Genetic Insert: RFXANK homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_RFXANK_1 and PX458_RFXANK_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified RFXANK. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86296 Copy
Species: Homo sapiens
Genetic Insert: ZNF792 homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_ZNF792_1 and PX458_ZNF792_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF792. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86282 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:5088; Vector Backbone:pBbA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25496359
Proper citation: RRID:Addgene_86202 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25496359
Comments: This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_86201 Copy
Species: Homo sapiens
Genetic Insert: HOMEZ homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_HOMEZ_1 and PX458_HOMEZ_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified HOMEZ. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86289 Copy
Species: Other
Genetic Insert: mTfp1
Vector Backbone Description: Vector Backbone:pUAS; Vector Types:zebrafish; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26838932
Proper citation: RRID:Addgene_86371 Copy
Species: Formosa B
Genetic Insert: FbGH30
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28213541
Proper citation: RRID:Addgene_86463 Copy
Species: Natronobacterium gregoryi
Genetic Insert: NgAgo
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28609472
Proper citation: RRID:Addgene_86435 Copy
Genetic Insert: PH(Btk)KD(Akt1)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28157504
Proper citation: RRID:Addgene_86636 Copy
Genetic Insert: Akt2(K14A/R25E)-Halo-PS
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28157504
Proper citation: RRID:Addgene_86632 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28174584
Comments: See Hahn et al., 2017
Proper citation: RRID:Addgene_86556 Copy
Species: E. coli
Genetic Insert: E. coli MutL(R95F)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851738
Proper citation: RRID:Addgene_86608 Copy
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