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Species: Synthetic
Genetic Insert: pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Vector Backbone Description: Backbone Marker:Joung Lab (DR274); Vector Backbone:DR274 (Addgene Plasmid #42250); Vector Types:Bacterial Expression, In vitro transcription (mRNA synthesis); Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_140082 Copy
Species: Aequorea victoria
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Proper citation: RRID:Addgene_140015 Copy
Species: Methanomethylophilus alvus Mx1201
Genetic Insert: Mx1201 PylRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30260624
Comments: Please note there is a T72A point mutation in Neo/KanR. Depositor confirms this does not affect function.
Proper citation: RRID:Addgene_140011 Copy
Species: Escherichia coli
Genetic Insert: EcoTyrRS
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37935196
Proper citation: RRID:Addgene_140018 Copy
Species: Other
Genetic Insert: FlaviA-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-FlaviA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140088 Copy
Species: Mus musculus
Genetic Insert: Tcf4B
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14667415
Proper citation: RRID:Addgene_14019 Copy
Species: Synthetic
Genetic Insert: sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_140096 Copy
Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.
Proper citation: RRID:Addgene_14015 Copy
Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140091 Copy
Species: Empty backbone
Genetic Insert: Empty backbone
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28525643
Proper citation: RRID:Addgene_140288 Copy
Vector Backbone Description: Backbone Marker:MIT; Vector Backbone:pLentiCrispr-V2-mOrange (V2mO); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32053639
Comments: Lenti Crispr/Cas9 V2 with mOrange fluorescent marker, to make lentiviral production visible and target cell transduction visible with mOrange.
The cloning method for inserting a guide RNA (gRNA) is: BsmB1.
Proper citation: RRID:Addgene_140206 Copy
Species: Homo sapiens
Genetic Insert: DNA damage response
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31991288
Proper citation: RRID:Addgene_140219 Copy
Species: Homo sapiens
Genetic Insert: eGFP-2A-Tau
Vector Backbone Description: Backbone Size:6468; Vector Backbone:AAV.CBA.WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31249873
Comments: Please Note: Plasmid contains a 152bp deletion in the backbone that removes the first 84bp of the ITR upstream of the CAG promoter. This deletion is not known to affect plasmid function. Plasmid 240284 (https://www.addgene.org/240284/) is an alternative version with a complete ITR.
Proper citation: RRID:Addgene_140424 Copy
Species: Homo sapiens
Genetic Insert: 5-HT sensor GRAB_5-HT1.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33821000
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.24.962282v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_140552 Copy
Species: Other
Genetic Insert: T7-lacO-TtgR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140391 Copy
Species: Homo sapiens
Genetic Insert: Smad2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9214507
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14042 Copy
Genetic Insert: dnaQ926 dam seqA emrR ugi CDA1 gIII
Vector Backbone Description: Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: Please note that Addgene's NGS found a 65bp insertion in the ori region as compared to the Depositor's sequence. The effect of this insertion is unknown.
Proper citation: RRID:Addgene_140446 Copy
Genetic Insert: Azurite
Vector Backbone Description: Backbone Size:6026; Vector Backbone:pBAD18-Cm; Vector Types:Bacterial Expression, Yeast/Bacteria optimized codons; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17115054
Proper citation: RRID:Addgene_14034 Copy
Species: Other
Genetic Insert: T7-lacO-TetR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140371 Copy
Genetic Insert: dCas9 & Csy4
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section.
Proper citation: RRID:Addgene_140664 Copy
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