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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCaRNA3-eIFiso4E120(+) Resource Report Resource Website |
RRID:Addgene_86092 | eIFiso4E | peach (Prunus persica) | Kanamycin | PMID:27565765 | Backbone Marker:Dr. Rao (University of California at Riverside); Backbone Size:11000; Vector Backbone:pCass4Rz; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:30 | 0 | ||
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pCAMBIA-FLS2prom::FLS2-3xmyc Resource Report Resource Website |
RRID:Addgene_86158 | FLS2 | Arabidopsis thaliana | Kanamycin | PMID:16510871 | Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:31 | 0 | ||
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pCAMBIA-FLS2prom::FLS2-3xmyc-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_86157 | FLS2 | Arabidopsis thaliana | Kanamycin | PMID:16510871 | Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:32 | 1 | ||
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pICSLUS0003OD Resource Report Resource Website |
RRID:Addgene_86176 | Kanamycin | Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:31 | 0 | |||||
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pICSLUS0004OD Resource Report Resource Website |
RRID:Addgene_86177 | Kanamycin | Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:32 | 0 | |||||
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pStI6 Resource Report Resource Website |
RRID:Addgene_8616 | Ribonuclease inhibitor StI | Saccharopolyspora erythraea, Strain NRRL 2338 | Kanamycin | PMID:14741361 | Production plasmid for ribonuclease inhibitor and barstar homolog StI from Saccharopolyspora erythraea. Structural gene for inhibitor StI from Saccharopolyspora erythraea strain NRRL 2338. N-terminal serine of wild type changed to glycine and 19 silent mutations improve production in E. coli. | Backbone Marker:Novagen; Backbone Size:5252; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | S2G point mutation; 19 silent mutations improve production in E. coli. | 2026-08-15 01:21:32 | 0 |
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pFETCh_ZNF644 Resource Report Resource Website |
RRID:Addgene_86264 | ZNF644 homology arms | Homo sapiens | Kanamycin | PMID:26355004 | This plasmid was co-transfected along with PX458_ZNF644_1 and PX458_ZNF644_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF644. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:32 | 0 | |
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pFETCh_KLF11 Resource Report Resource Website |
RRID:Addgene_86269 | KLF11 homology arms | Homo sapiens | Kanamycin | PMID:26355004 | This plasmid was co-transfected along with PX458_KLF11_1 and PX458_KLF11_2 into HepG2 cells, and genomic editing was verified by PCR. The donor nucleotide sequence in the final codon of the KLF11 gene is modified to discourage sgRNA recognition, while retaining the native amino acid sequence. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified KLF11. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:32 | 0 | |
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pFETCh_RFXANK Resource Report Resource Website |
RRID:Addgene_86296 | RFXANK homology arms | Homo sapiens | Kanamycin | PMID:26355004 | This plasmid was co-transfected along with PX458_RFXANK_1 and PX458_RFXANK_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified RFXANK. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:32 | 0 | |
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pFETCh_ZNF792 Resource Report Resource Website |
RRID:Addgene_86282 | ZNF792 homology arms | Homo sapiens | Kanamycin | PMID:26355004 | This plasmid was co-transfected along with PX458_ZNF792_1 and PX458_ZNF792_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF792. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:32 | 0 | |
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pBbA5k-AcrB/AcrD/AcrFa, ABO_0964 Resource Report Resource Website |
RRID:Addgene_86202 | Efflux pump | Kanamycin | PMID:25496359 | Backbone Size:5088; Vector Backbone:pBbA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:31 | 0 | |||
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pBbA5k-Maqu_3494 Resource Report Resource Website |
RRID:Addgene_86201 | Efflux pump | Kanamycin | PMID:25496359 | This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function. | Vector Backbone:pBbA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:31 | 0 | ||
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pFETCh_HOMEZ Resource Report Resource Website |
RRID:Addgene_86289 | HOMEZ homology arms | Homo sapiens | Kanamycin | PMID:26355004 | This plasmid was co-transfected along with PX458_HOMEZ_1 and PX458_HOMEZ_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified HOMEZ. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. | Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:33 | 0 | |
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pUAS-MmTFP13xmyc Resource Report Resource Website |
RRID:Addgene_86371 | mTfp1 | Other | Kanamycin | PMID:26838932 | Vector Backbone:pUAS; Vector Types:zebrafish; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:33 | 0 | ||
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pET28a(+)/FbGH30 Resource Report Resource Website |
RRID:Addgene_86463 | FbGH30 | Formosa B | Kanamycin | PMID:28213541 | Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:34 | 0 | ||
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Flag-nls-NgAgo-GK Resource Report Resource Website |
RRID:Addgene_86435 | NgAgo | Natronobacterium gregoryi | Kanamycin | PMID:28609472 | Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:34 | 0 | ||
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pmCherry-PH(Btk)KD(Akt1) Resource Report Resource Website |
RRID:Addgene_86636 | PH(Btk)KD(Akt1) | Kanamycin | PMID:28157504 | Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:36 | 0 | |||
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pEGFP-Akt2(K14A/R25E)-Halo-PS Resource Report Resource Website |
RRID:Addgene_86632 | Akt2(K14A/R25E)-Halo-PS | Kanamycin | PMID:28157504 | Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:21:36 | 0 | |||
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pUB-Cas9 Resource Report Resource Website |
RRID:Addgene_86556 | Cas9 | Synthetic | Kanamycin | PMID:28174584 | See Hahn et al., 2017 | Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin | Cas9 is codon optimized for C. reinhardtii | 2026-08-15 01:21:35 | 0 |
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pRAF-MutL(R95F) Resource Report Resource Website |
RRID:Addgene_86608 | E. coli MutL(R95F) | E. coli | Kanamycin | PMID:27851738 | Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | R95F | 2026-08-15 01:21:36 | 0 |
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