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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pMX155
 
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1+ mentions
RRID:Addgene_29481 Kif2A-alpha Mus musculus Kanamycin PMID:15883193 Depositor believes the mutation in Kif2a is the results of either errors in database sequences or polymorphisms. Backbone Marker:Clontech; Backbone Size:4706; Vector Backbone:PEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin E140K 2026-08-15 01:13:20 1
pCool-mRbx1-His-hCul1-CTP
 
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1+ mentions
RRID:Addgene_29519 Rbx1 CUL1 Homo sapiens Ampicillin Backbone Size:5136; Vector Backbone:pCool; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:20 1
pFB-HTB-ASK1
 
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1+ mentions
RRID:Addgene_29517 ASK1 Arabidopsis thaliana Ampicillin PMID:20927106 Backbone Size:0; Vector Backbone:pFB-HTB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:20 1
pFB-GTE-COI1
 
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1+ mentions
RRID:Addgene_29516 COI1 Arabidopsis thaliana Ampicillin PMID:20927106 Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pFastBac-GTE; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Note that the first AA in TEV site is R, not E 2026-08-15 01:13:20 1
416GPD-FUS-YFP
 
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1+ mentions
RRID:Addgene_29594 FUS Homo sapiens Ampicillin PMID:21541367 Backbone Marker:Susan Lindquist (Addgene plasmid # 14220); Backbone Size:8203; Vector Backbone:pAG416GPD-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:21 1
MKK4
 
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1+ mentions
RRID:Addgene_29579 MKK4 Homo sapiens Ampicillin PMID:23790491 Starting Met removed. Ligation-independent-cloning. Backbone Size:5961; Vector Backbone:pMCSG10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:19 2
MKK7
 
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1+ mentions
RRID:Addgene_29576 MKK7 Mus musculus Ampicillin PMID:23790491 Ligation-independent cloning. Backbone Size:5961; Vector Backbone:pMCSG10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Starting Met removed 2026-08-15 01:13:19 1
tol2-mpx-Dendra2
 
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1+ mentions
RRID:Addgene_29574 mpx-Dendra 2 Dendronephthya Ampicillin PMID:21248150 mpx is a promoter for neutrophil expression and was called zMPO in the associated publication Backbone Size:3000; Vector Backbone:tol2; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:13:21 2
426Gal-FUS-YFP
 
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1+ mentions
RRID:Addgene_29592 FUS Homo sapiens Ampicillin PMID:21541367 Backbone Marker:Susan Lindquist (Addgene plasmid # 14227); Backbone Size:8126; Vector Backbone:pAG426Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:21 1
pcDNA3/FLHIP1
 
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1+ mentions
RRID:Addgene_29587 Huntingtin-interacting protein 1 Homo sapiens Ampicillin PMID:12163454 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:21 1
Erk2
 
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1+ mentions
RRID:Addgene_29582 Erk2 Rattus norvegicus Ampicillin PMID:23790491 Ligation-independent-cloning. Backbone Size:5286; Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Starting Met removed from Erk2 2026-08-15 01:13:19 1
pET His6 ProteinG TEV LIC cloning vector (2P-T)
 
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1+ mentions
RRID:Addgene_29713 Ampicillin This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). 2P-T has a TEV-cleavable N-terminal His6-Protein G fusion tag. Protein G can improve the expression and solubility of your target protein. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:4975; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:20 2
pET His6 Thioredoxin TEV LIC cloning vector (2T-T)
 
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1+ mentions
RRID:Addgene_29712 Ampicillin This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). 2T-T has a TEV-cleavable N-terminal His6-thioredoxin fusion tag. Thioredoxin can improve the solubility of your target protein by promoting correct disulfide bond formation. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:5131; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:23 1
pET His6 Mocr TEV LIC cloning vector (2O-T)
 
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1+ mentions
RRID:Addgene_29710 Ampicillin This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). 2O-T has a TEV-cleavable N-terminal His6-Mocr fusion tag. Mocr can improve the expression and solubility of your target protein. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:5908; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:20 6
426Gal-FUS-RRM Mutant-YFP
 
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1+ mentions
RRID:Addgene_29608 FUS RRM mutant Homo sapiens Ampicillin PMID:21541367 Backbone Marker:Susan Lindquist (Addgene plasmid # 14227); Backbone Size:8126; Vector Backbone:pAG426Gal-ccdB-EYFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin F305L, F341L, F359L and F368L 2026-08-15 01:13:21 1
pET His6 MBP prescission LIC cloning vector (HMPKS)
 
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1+ mentions
RRID:Addgene_29721 None Kanamycin This plasmid is an empty vector. Your gene can be inserted with a SLIC cloning protocol. Prescission is a highly specific protease that cleaves LEVLFQ/GP. It is generally more active than TEV protease, and many users have found that when their protein inhibits TEV cleavage, switching to a prescission vector has proved worthwhile. To clone into this vector, add SLIC tags to the 5' end of your PCR primers. Forward - 5'GTGCTGTTCCAGGGTCCGAAT3' Reverse - 5'TGGTGGTGGTGGTGCTCGA(TTA)3' Linearize the plasmid with SspI and XhoI, then gel purify. There is a 1650 bp stuffer sequence that will be visible on a gel. When digesting the DNA with T4 polymerase, use no nucleotides for either your insert or the linearized vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:8106; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:23 4
pET His6 Sumo TEV LIC cloning vector (1S)
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_29659 None Kanamycin This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV-cleavable His6 fusion tag on its N-terminus. Sumo can enhance the expression and solubility of your protein of interest. To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/macrolab/ Backbone Size:5661; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:20 36
pET His6 Mocr TEV LIC cloning vector (1O)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_29658 None Kanamycin This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV-cleavable His6 fusion tag on its N-terminus. Mocr can enhance the expression and solubility of your protein of interest. To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:5712; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:22 1
pET His6 MBP TEV LIC cloning vector (1M)
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_29656 None Kanamycin This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV-cleavable His6 fusion tag on its N-terminus. MBP can enhance your protein's solubility and expression. It can also be used as an affinity tag. To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:6465; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:20 48
pET His6 GST TEV LIC cloning vector (1G)
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_29655 None Kanamycin This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV-cleavable His6 fusion tag on its N-terminus. GST can be used to enhance your protein's expression and solubility. It can also be used as an affinity tag. To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:6015; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:22 15

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