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Species: Mus musculus
Genetic Insert: ROSA26-5' insert
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3027; Vector Backbone:pBluescript KS (-); Vector Types:for use with mouse targeting vector pROSA26-1; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9916792
Comments: Probe for detecting targeted integrations by Southern blots. It is a small probe so users should wash at low stringency, eg. 1xSSC at 65 degrees.
We recommend you order this plasmid along with pROSA26-1 and SAbgeo as a "ROSA26 targeting kit".
For more information visit -
http://research.mssm.edu/soriano/lab
Proper citation: RRID:Addgene_21715 Copy
Species: Mus musculus
Genetic Insert: ROSA26 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2911; Vector Backbone:pBluescript KS (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9108056
Comments: Fragment 5' of exon 1; promoter fragment for driving expression.
For more information visit -
http://research.mssm.edu/soriano/lab
Proper citation: RRID:Addgene_21710 Copy
Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16973439
Proper citation: RRID:Addgene_21794 Copy
Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16973439
Comments: Vector may be pcDNA3.1
Proper citation: RRID:Addgene_21793 Copy
Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5006; Vector Backbone:pGEX.KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16973439
Comments: We DO NOT have a good record for how this plasmid was constructed.
The best guess is that Jin Liu used the m1.9Bam.1S primer and T7
to PCR the CDS + 3'UTR from m1.9pBS and ligated the BamH1-Xba1
fragment in frame with GST in pGEX.KG
Proper citation: RRID:Addgene_21792 Copy
Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Weinberg lab; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16973439
Comments: Addgene sequence shows no Myc tag in this construct.
Proper citation: RRID:Addgene_21797 Copy
Species: Mus musculus
Genetic Insert: PERK
Vector Backbone Description: Backbone Marker:Plasmid available at Addgene (#1764); Backbone Size:5150; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: 9E10-tagged wildtype mouse PERK in the pro-retroviral vector pBABEpuro
Mammalian expression plasmid encoding wildtype mouse PERK fused to 9E10 epitope at C-term.
Proper citation: RRID:Addgene_21819 Copy
Species: Mus musculus
Genetic Insert: PERK (537-1114) K618R
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9930704
Comments: Mouse PERK (537-1114), K618R mutant, 9E10-tagged at C-terminus, fused to GST, bacterial expression vector, pGEX4T-1
Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.
Proper citation: RRID:Addgene_21818 Copy
Species: Mus musculus
Genetic Insert: PERK
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Modified pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mouse PERK Full-length cDNA in modified pBSKS plasmid.
Proper citation: RRID:Addgene_21821 Copy
Species: Mus musculus
Genetic Insert: PERK K618A
Vector Backbone Description: Backbone Marker:Plasmid available at Addgene (#1764); Backbone Size:5150; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: 9E10-tagged mouse PERK K618A mutant in the pro-retroviral vector pBABEpuro
Mammalian expression plasmid encoding kinase dead K618A mutant mouse PERK fused to 9E10 epitope at C-term.
Note: Addgene has yet to be able to sequence the C-terminal Myc tag, therefore we cannot guarantee that is present.
Proper citation: RRID:Addgene_21820 Copy
Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mouse IRE1b, wild-type, full-length coding cDNA in bluescript SK, no tags, assembled from lambda-phage clones 37 and 26
Proper citation: RRID:Addgene_21878 Copy
Species: Mus musculus
Genetic Insert: GCN2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11106749
Comments: Used to raise antisera to GCN2.
Proper citation: RRID:Addgene_21876 Copy
Species: Mus musculus
Genetic Insert: BCL2L11
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21959 Copy
Species: Mus musculus
Genetic Insert: BCL2L11
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21958 Copy
Species: Mus musculus
Genetic Insert: neuropilin 1
Vector Backbone Description: Backbone Marker:Pelicci's laboratory; Backbone Size:0; Vector Backbone:Pinco; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19175293
Proper citation: RRID:Addgene_21937 Copy
Species: Mus musculus
Genetic Insert: neuropilin 1
Vector Backbone Description: Backbone Marker:Pelicci's laboratory; Backbone Size:0; Vector Backbone:Pinco; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19175293
Proper citation: RRID:Addgene_21936 Copy
Species: Mus musculus
Genetic Insert: neuropilin 1
Vector Backbone Description: Backbone Marker:Pelicci's laboratory; Backbone Size:0; Vector Backbone:Pinco; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19175293
Proper citation: RRID:Addgene_21935 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Size:4534; Vector Backbone:pSRa; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: pSRa and its associated helper plasmids are described in: Muller, AJ et al 1992 Mol Cell Biol 11:941, and helper plasmids were obtained from the lab of Dr. Owen Witte
Proper citation: RRID:Addgene_21912 Copy
Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Mouse IRE1b, delta C-term, 9E10-tagged at C-term, assembled from lambda-phage clone 37, mammalian expression vector, pCDNA3.
Proper citation: RRID:Addgene_21882 Copy
Species: Mus musculus
Genetic Insert: Chd1-i4
Vector Backbone Description: Backbone Size:7558; Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19587682
Comments: Chd1 RNAi4 sequence - 5'-GTGCTACTACAACCATTTA
Proper citation: RRID:Addgene_21908 Copy
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