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Species: Homo sapiens
Genetic Insert: FnR.0.6.2-Y78R
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_84798 Copy
Species: Homo sapiens
Genetic Insert: FnR.0.6.2-Y78I
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_84791 Copy
Species: Homo sapiens
Genetic Insert: FnR.0.6.2-Y78N
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_84795 Copy
Species: Homo sapiens
Genetic Insert: FnR.0.6.2-Y78M
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_84794 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PdX gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85084 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin reductase
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PdR gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85083 Copy
Species: Synthetic
Genetic Insert: The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: A synthetic gene of PdR was inserted at the NdeI site of p1G108CP20X (Plasmid #85096) using In-Fusion cloning kit.
Proper citation: RRID:Addgene_85086 Copy
Species: Synthetic
Genetic Insert: PCNA3 fused to P450cam
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The plamid was obtained from pGS-3 (Plasmid #85094) and pBsaP450camGG (Plasmid #85101) by Golden Gate assembly.
Proper citation: RRID:Addgene_85087 Copy
Species: Synthetic
Genetic Insert: DNA polymerase sliding clamp 3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5329; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85093 Copy
Species: Synthetic
Genetic Insert: DNA polymerase sliding clamp 3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85094 Copy
Species: Homo sapiens
Genetic Insert: HLA.A2
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24293164
Proper citation: RRID:Addgene_85161 Copy
Genetic Insert: RpaR, LasI
Vector Backbone Description: Vector Backbone:pTD103; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27172092
Proper citation: RRID:Addgene_85160 Copy
Vector Backbone Description: Vector Backbone:pBBR1MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8529885
Comments: Vector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not controllable with IPTG, due to absence of lacI repressor gene on the plasmid. (chromosomal lacI does not provide sufficient repression).
MCS: Has unique KpnI, ApaI, XhoI, HincII, SalI, ClaI, HindIII, EcoRV, EcoRI, SmaI, BamHI, SpeI, XbaI, SacII, SacI sites for cloning
Proper citation: RRID:Addgene_85168 Copy
Species: Mus musculus
Genetic Insert: mouse NFH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4344; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19468066
Comments: Please cite Çolakoğlu & Brown (2009), "Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing", Journal of Cell Biology 185: 769-777. This plasmid was used in that study but was inadvertently not described in the Methods.
Proper citation: RRID:Addgene_85178 Copy
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:Ptrc-YFP expressed from a NS2 vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25127213
Proper citation: RRID:Addgene_85123 Copy
Species: Homo sapiens
Genetic Insert: human full-length METTL20
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt METTL20, full-length, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85115 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: EFM3
Vector Backbone Description: Backbone Marker:EMD biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25231979
Proper citation: RRID:Addgene_85118 Copy
Species: Homo sapiens
Genetic Insert: human full-length ETFbeta
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt ETF beta, full-length, cloned into NdeI-HindIII sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85110 Copy
Species: Homo sapiens
Genetic Insert: human mature (delta25) MCAD
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt MCAD, delta 25, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85112 Copy
Species: Homo sapiens
Genetic Insert: human mature (delta44) GCDH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25416781
Comments: Homo sapiens wt GCDH, delta 44, cloned into NdeI-NotI sites of pET28a, contains a N-terminal His-tag
Proper citation: RRID:Addgene_85113 Copy
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