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Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Size:4767; Vector Backbone:pcDNA1.1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8650547
Comments: Mammalian expression plasmid encoding S78A & S81A mutant mouse CHOP fused to DNA-binding domain of yeast Gal4.
Proper citation: RRID:Addgene_21901 Copy
Species: Mus musculus
Genetic Insert: Chd1-i1
Vector Backbone Description: Backbone Size:7558; Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19587682
Comments: Chd1 RNAi1 sequence 5'-ACATTATGATGGAGCTAAA
Proper citation: RRID:Addgene_21904 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Size:4534; Vector Backbone:pSRa; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: pSRa and its associated helper plasmids are described in: Muller, AJ et al 1992 Mol Cell Biol 11:941, and helper plasmids were obtained from the lab of Dr. Owen Witte
NB: From Addgene's sequencing results, there is a 6bp insertion into the insert of CHOP4 that is not in the depositor's sequence.
Proper citation: RRID:Addgene_21903 Copy
Species: Mus musculus
Genetic Insert: FGF8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7768185
Comments: Mouse FGF8 cDNA splice variant 1(B) clone was isolated from an E7.5 cDNA library. The insert contains the entire coding region and 3' UTR. The SmaI site is in the FGF8 5'UTR 16bp upstream of the translation start site. The 3' XhoI site is from the vector MCS. The FGF8 variant 1(B) sequence is ~30bp longer than the variant 4(A) sequence.
For antisense probe linearize with BamHI and use T7 Polymerase. The transcript is ~875bp.
Proper citation: RRID:Addgene_22090 Copy
Species: Mus musculus
Genetic Insert: FGF8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7768185
Comments: Mouse FGF8 cDNA splice variant 4(A) clone was isolated from an E7.5 cDNA library. The insert contains the entire coding region and 3' UTR. The SmaI site is in the FGF8 5'UTR 16bp upstream of the translation start site. The 3' XhoI site is from the vector MCS. The FGF8 variant 4(A) sequence is ~30bp shorter than the variant 1(B) sequence.
For antisense probe linearize with BamHI and use T7 Polymerase.The transcript is ~840bp.
Proper citation: RRID:Addgene_22088 Copy
Species: Mus musculus
Genetic Insert: FGF4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2318343
Comments: Full length coding region of K-FGF, PCR clone. The insert can be cut out with EcoRI and BamHI.
Proper citation: RRID:Addgene_22085 Copy
Species: Mus musculus
Genetic Insert: FGF5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin
Comments: Full length coding region, PCR clone. The insert can be cut out with EcoRI and BamHI.
Proper citation: RRID:Addgene_22087 Copy
Species: Mus musculus
Genetic Insert: Sprouty 1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10498682
Comments: Mouse Sprouty1 cDNA clone was isolated from Lambda Zap mouse cDNA library. The insert contains the complete coding region as well as some 5' and 3' UTR sequences. The 4kb vector is presumed to be pBluescript with extra sequences from Lambda Zap plasmid.
For antisense probe, linearize the plasmid with EcoRI and use T7 Polymerase. The transcript should be approximately 1.5kb.
Proper citation: RRID:Addgene_22091 Copy
Species: Mus musculus
Genetic Insert: mouse PrP ORF insert
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBTIIKS(+); Vector Types:construction of targeting vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19709627
Proper citation: RRID:Addgene_22109 Copy
Species: Mus musculus
Genetic Insert: mouse PrP ORF insert
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBTIIKS(+); Vector Types:construction of targeting vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19709627
Proper citation: RRID:Addgene_22106 Copy
Species: Mus musculus
Genetic Insert: Proline Serine Rich protein 1
Vector Backbone Description: Backbone Size:7241; Vector Backbone:PiggyBac-GW-IRES-Blast; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39562138
Comments: Please visit https://doi.org/10.1101/2024.07.31.606086 for bioRxiv preprint.
Proper citation: RRID:Addgene_226172 Copy
Species: Mus musculus
Genetic Insert: KIF 13a S1371D
Vector Backbone Description: Vector Backbone:pBeta-Actin-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38598291
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_224417 Copy
Species: Mus musculus
Genetic Insert: Rab5
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pMRX-IPU; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39288101
Comments: Please note: This plasmid contains a 75bp deletion in the backbone and LTR. This deletion is not expected to impact plasmid function.
Proper citation: RRID:Addgene_228139 Copy
Species: Mus musculus
Genetic Insert: Ar
Vector Backbone Description: Vector Backbone:pBK; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39405910
Proper citation: RRID:Addgene_227098 Copy
Species: Mus musculus
Genetic Insert: TMEM63A/CSC1-like protein 1 isoform 1
Vector Backbone Description: Vector Backbone:peGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38370744
Comments: Please visit https://doi.org/10.1101/2024.02.04.578431 for bioRxiv preprint.
Proper citation: RRID:Addgene_228592 Copy
Species: Mus musculus
Genetic Insert: TMEM16F/anoctamin 6 (Ano6), transcript variant 2
Vector Backbone Description: Vector Backbone:peGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38370744
Comments: Please visit https://doi.org/10.1101/2024.02.04.578431 for bioRxiv preprint.
Proper citation: RRID:Addgene_228578 Copy
Species: Mus musculus
Genetic Insert: myosin heavy chain 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3016; Vector Backbone:pGEM-Teasy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36517457
Proper citation: RRID:Addgene_192841 Copy
Species: Mus musculus
Genetic Insert: myosin heavy chain 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3016; Vector Backbone:pGEM-Teasy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36517457
Proper citation: RRID:Addgene_192843 Copy
Species: Mus musculus
Genetic Insert: myosin heavy chain 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3016; Vector Backbone:pGEM-Teasy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36517457
Proper citation: RRID:Addgene_192842 Copy
Species: Mus musculus
Genetic Insert: myosin heavy chain 7
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3016; Vector Backbone:pGEM-Teasy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36517457
Proper citation: RRID:Addgene_192849 Copy
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