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Species: Vibrio cholera, Tatumella citrea
Genetic Insert: Mini-Tn6677, T7lac-GDH gene cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32551502
Proper citation: RRID:Addgene_140629 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Transposon; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32551502
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140624 Copy
Species: Synthetic
Genetic Insert: 24xMS2V7
Vector Backbone Description: Backbone Marker:Genescript; Backbone Size:2749; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131164
Proper citation: RRID:Addgene_140705 Copy
Species: Aequorea victoria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:4942; Vector Backbone:pBBR1MCS4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17207855
Proper citation: RRID:Addgene_14076 Copy
Species: Phage T4
Genetic Insert: Rnl2(1-249)
Vector Backbone Description: Backbone Marker:Novagn; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_14072 Copy
Genetic Insert: Bacterial luciferase
Vector Backbone Description: Backbone Size:4980; Vector Backbone:pBBR1MCS4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17207855
Proper citation: RRID:Addgene_14081 Copy
Genetic Insert: Bacterial luciferase
Vector Backbone Description: Backbone Size:4982; Vector Backbone:pBBR1MCS4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17207855
Proper citation: RRID:Addgene_14080 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1(F74G)-P2A-mAID-EGFP-NES
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140730 Copy
Species: Synthetic
Genetic Insert: Cas9m4-KRAB-MeCP2
Vector Backbone Description: Backbone Marker:Andrea Califano; Vector Backbone:PB-TRE-dCas9-KRAB-MeCP2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: This is a lentiviral vector (modified in #122205; originally from Weissman lab #85969) with a Tet-ON 3G dCas9m4-KRAB-MeCP2 (Church lab; #63800). Note: the vector has been modified to use blasticidin selection.
Proper citation: RRID:Addgene_140690 Copy
Genetic Insert: Bacterial luciferase
Vector Backbone Description: Backbone Size:5681; Vector Backbone:pAKgfp1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17207855
Proper citation: RRID:Addgene_14083 Copy
Species: Homo sapiens
Genetic Insert: Vav1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMX-uGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16709244
Comments: The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP.
Proper citation: RRID:Addgene_14557 Copy
Species: Homo sapiens
Genetic Insert: Vav1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMX-uGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16709244
Comments: The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP.
Proper citation: RRID:Addgene_14558 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp4
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725300.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145495 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725304.1
Proper citation: RRID:Addgene_145584 Copy
Species: Homo sapiens
Genetic Insert: cdc42 Q61L
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16546099
Proper citation: RRID:Addgene_14568 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724390.1
Proper citation: RRID:Addgene_145730 Copy
Species: Bos taurus
Genetic Insert: PIK3CA E545K
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16322248
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
Proper citation: RRID:Addgene_14571 Copy
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16322248
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA). The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
Proper citation: RRID:Addgene_14570 Copy
Species: Homo sapiens
Genetic Insert: kappa-myc-dK-2xG4S-TMst
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5358; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786268
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
For more information about this plasmid, please see the associated publication (PMID: 32786268).
Proper citation: RRID:Addgene_145766 Copy
Species: HIV-1
Genetic Insert: Capsid
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:2863; Vector Backbone:pTZ19U; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33033190
Proper citation: RRID:Addgene_145784 Copy
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