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Species: Mus musculus
Genetic Insert: Grin2a
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124863 Copy
Species: Mus musculus
Genetic Insert: Gria1
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124867 Copy
Species: Mus musculus
Genetic Insert: Grin1
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124865 Copy
Species: Mus musculus
Genetic Insert: HH7-8 TCRa P2A TCRb
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124991 Copy
Species: Mus musculus
Genetic Insert: HH7-9 TCRa P2A TCRb
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124992 Copy
Species: Mus musculus
Genetic Insert: HH7-6 TCRa P2A TCRb
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124990 Copy
Species: Mus musculus
Genetic Insert: HH7-11 TCRa P2A TCRb
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124993 Copy
Species: Mus musculus
Genetic Insert: HH7-2 TCRa Tg
Vector Backbone Description: Vector Backbone:pTa; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124997 Copy
Species: Mus musculus
Genetic Insert: Tbkbp1
Vector Backbone Description: Backbone Marker:Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557; Vector Backbone:pCLXSN(GFP)-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30022064
Proper citation: RRID:Addgene_125179 Copy
Species: Mus musculus
Genetic Insert: Tbkbp1
Vector Backbone Description: Backbone Marker:Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557; Vector Backbone:pCLXSN(GFP)-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30022064
Proper citation: RRID:Addgene_125177 Copy
Species: Mus musculus
Genetic Insert: RLF-CAAX
Vector Backbone Description: Backbone Size:5137; Vector Backbone:pBabe HAII; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12592 Copy
Species: Mus musculus
Genetic Insert: GB1 domain of protein G as N terminal tag, with Bcl2 as insert
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9385638
Comments: In GEV1, a noncleavable linker (Pro-Gly-Gly-Pro-Ala-Ser) is encoded between the GBl domain and the N-terminus of the protein of interest. Genes can be inserted into this vector using Nhe I and Xho I cloning sites, and, if no stop codons are introduced at the 5' end of an insert, the vector encodes a C-terminal poly His-tag.
Also see: "A rapid method to attain isotope labeled small soluble peptides for NMR studies", Journal of Biomolecular NMR, Vol 26, No 3, July 2003.
Proper citation: RRID:Addgene_12615 Copy
Species: Mus musculus
Genetic Insert: NIPBL
Vector Backbone Description: Backbone Size:4063; Vector Backbone:pN3-3xFlag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The plasmid encodes the 2798 amino acid full-length murine NIPBL
Proper citation: RRID:Addgene_107716 Copy
Species: Mus musculus
Genetic Insert: whole pre-mmu-miR-30a gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5699; Vector Backbone:pcDNA6.2-GW/EmGFP; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Spectinomycin
Comments: Whole pre-mmu-miR-30a gene (miRBase ID: MI0000144) was cloned into the plasmid. It generates a pre-mmu-miR-30a, which matures to mmu-miR-30a-5p and potentially to mmu-miR-30a-3p. Plasmid constructed by Marc Beltrà in the Penna lab.
Proper citation: RRID:Addgene_107791 Copy
Species: Mus musculus
Genetic Insert: mouse Beclin-1 (BECN1)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30089251
Proper citation: RRID:Addgene_107937 Copy
Species: Mus musculus
Genetic Insert: Egr3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: A Sal I site was introduced before the HindIII site to further transfer to pBabe.
Proper citation: RRID:Addgene_107998 Copy
Species: Mus musculus
Genetic Insert: mCD44v8
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS results identified V35A, S229N, M492I, K493N, and I494del variants compared to the NCBI reference. These are polymorphisms that should not affect plasmid function according to the depositing lab.
Proper citation: RRID:Addgene_107972 Copy
Species: Mus musculus
Genetic Insert: mCD44v6
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS results identified NCBI reference X66083.1 as closest match, but encoded protein contains the following variations: T8I, deletion of amino acids 263-304 and 369-408, V409M. These differences should not affect plasmid function according to the depositing lab.
Proper citation: RRID:Addgene_107970 Copy
Species: Mus musculus
Genetic Insert: Egr2
Vector Backbone Description: Backbone Size:5086; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: The Egr2 gene was transferred from pcDNA3.1-Egr2 (Addgene plasmid 107997) to the pBabe(puro) retroviral vector using EcoRI and SalI sites.
Proper citation: RRID:Addgene_108097 Copy
Species: Mus musculus
Genetic Insert: Vmn2r1-D-tauGFP-pA-ACNF
Vector Backbone Description: Backbone Marker:Agilent Technologies/Stratagene; Backbone Size:2900; Vector Backbone:pBluescript II SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29465786
Proper citation: RRID:Addgene_108080 Copy
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