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Species: Homo sapiens
Genetic Insert: human UVRAG
Vector Backbone Description: Vector Backbone:pmCherry-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25533187
Comments: IMAGE: 6186860
Proper citation: RRID:Addgene_86745 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5438; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27633000
Proper citation: RRID:Addgene_86775 Copy
Species: Homo sapiens
Genetic Insert: Rab12
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25925668
Proper citation: RRID:Addgene_86787 Copy
Species: Homo sapiens
Genetic Insert: Rab12
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25925668
Proper citation: RRID:Addgene_86788 Copy
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27562163
Comments: Skylan was generated by mutating H62L of mEos3.1
Proper citation: RRID:Addgene_86785 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP_Frt_KO
Vector Backbone Description: Vector Backbone:pMLS252; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28646043
Proper citation: RRID:Addgene_86734 Copy
Species: Homo sapiens
Genetic Insert: U6-gRNA-TAZ
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pCR-Blunt-II; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27929521
Proper citation: RRID:Addgene_86688 Copy
Vector Backbone Description: Backbone Size:7273; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired.
To clone into this vector, add LIC tags to the 5' end of your PCR primers.
Forward - 5' TACTTCCAATCCAATGCA 3'
Reverse - 5' CTCCCACTACCAATGCC 3'
Do NOT include a stop codon with your reverse primer.
Linearize the plasmid with SspI, then gel purify.
When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector.
More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
Proper citation: RRID:Addgene_86930 Copy
Species: Homo sapiens
Genetic Insert: UBL4A
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27867533
Proper citation: RRID:Addgene_86922 Copy
Vector Backbone Description: Backbone Size:7987; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired.
To clone into this vector, add LIC tags to the 5' end of your PCR primers.
Forward - 5' TACTTCCAATCCAATGCA 3'
Reverse - 5' CTCCCACTACCAATGCC 3'
Do NOT include a stop codon with your reverse primer.
Linearize the plasmid with SspI, then gel purify.
When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector.
More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
Proper citation: RRID:Addgene_86927 Copy
Species: Eschericia coli
Genetic Insert: YedK
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29020633
Proper citation: RRID:Addgene_86884 Copy
Species: Homo sapiens
Genetic Insert: HOXC13
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12761847
Comments: Tkatchenko et al Development 128: 1547-1558 (2001)
Proper citation: RRID:Addgene_8679 Copy
Species: Homo sapiens
Genetic Insert: FLAG-HaloTag-TERT Exon 1 and part of Exon 2
Vector Backbone Description: Vector Backbone:pFastBac; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27523609
Proper citation: RRID:Addgene_86843 Copy
Vector Backbone Description: Backbone Size:7267; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired.
To clone into this vector, add LIC tags to the 5' end of your PCR primers.
Forward - 5' TACTTCCAATCCAATGCA 3'
Reverse - 5' CTCCCACTACCAATGCC 3'
Do NOT include a stop codon with your reverse primer.
Linearize the plasmid with SspI, then gel purify.
When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector.
More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
Proper citation: RRID:Addgene_86933 Copy
Species: Mus musculus
Genetic Insert: postLib-mRFP-4xboxB
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28449067
Proper citation: RRID:Addgene_87006 Copy
Species: Mus musculus
Genetic Insert: preCLAP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28449067
Proper citation: RRID:Addgene_87003 Copy
Species: Mus musculus
Genetic Insert: postCLAP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28449067
Proper citation: RRID:Addgene_87004 Copy
Species: Staphylococcus aureus
Genetic Insert: S.aureus SrtA 5M
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET30b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28088451
Comments: Pentamutant sortase A was originally described by David R. Liu's laboratory (Harvard University) in: A General Strategy for the Evolution of Bond-Forming Enzymes Using Yeast Display. Chen I, Dorr BM, Liu DR. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404 (PubMed ID: 21697512). As noted in this publication, the cloned sortase A gene lacks the N-terminal 59 amino acids, which do not impact catalytic activity, but these amino acids are still included in the numbering for the mutations.
Proper citation: RRID:Addgene_86962 Copy
Species: Yersinia enterocolitica
Genetic Insert: irp3 WT
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23066849
Comments: Biochemistry 55 (2016) 5423-5433
Proper citation: RRID:Addgene_87106 Copy
Species: Pseudomonas aeruginosa PAO1
Genetic Insert: pchG WT
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27601130
Proper citation: RRID:Addgene_87107 Copy
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