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Species: Oryza Sativa
Genetic Insert: Pong TPase L418A, L420A
Vector Backbone Description: Vector Backbone:pAG415 GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20226077
Proper citation: RRID:Addgene_145795 Copy
Species: Homo sapiens
Genetic Insert: ATR shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14582 Copy
Species: Homo sapiens
Genetic Insert: ATM shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14581 Copy
Species: Homo sapiens
Genetic Insert: human TMPRSS2 variant 1
Vector Backbone Description: Vector Backbone:RRL.sin.cPPT.SFFV/IRES-neo.WPRE. ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514628
Comments: NM_001135099 with 1 mutation G8V
Proper citation: RRID:Addgene_145843 Copy
Vector Backbone Description: Vector Backbone:pT2AL200R175-CAGGS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32456235
Proper citation: RRID:Addgene_145813 Copy
Species: Homo sapiens
Genetic Insert: cyclin T1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX 2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9990016
Comments: There is a H519R mutation in the ORF.
Proper citation: RRID:Addgene_14607 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3260352
Proper citation: RRID:Addgene_14614 Copy
Species: Homo sapiens
Genetic Insert: B1AR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10535961
Comments: Addgene Sanger sequencing found G389R in the B1AR translation
Proper citation: RRID:Addgene_14698 Copy
Species: Mus musculus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3300; Vector Backbone:pQE9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11740563
Proper citation: RRID:Addgene_14667 Copy
Species: Homo sapiens
Genetic Insert: V1H
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7500; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12058068
Comments: For two-hybrid assay.
Proper citation: RRID:Addgene_14665 Copy
Species: Mus musculus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3300; Vector Backbone:pQE9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10811609
Comments: Addgene NGS results identified a V206 variant in p97 that matches NCBI reference CAA78412.1.
Proper citation: RRID:Addgene_14666 Copy
Species: Mus musculus
Genetic Insert: ZP1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7635043
Proper citation: RRID:Addgene_14644 Copy
Species: HIV
Genetic Insert: Tat
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121429
Comments: Mutant Tat gene: C30G is in the activation domain of Tat and renders Tat inactive without affecting its RNA-binding ability or protein expression levels.
Proper citation: RRID:Addgene_14656 Copy
Vector Backbone Description: Backbone Size:8174; Vector Backbone:pLKO.3G; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.
pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.
Proper citation: RRID:Addgene_14748 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta K85A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8382613
Comments: Nru I site is added to the K->A mutation
Proper citation: RRID:Addgene_14755 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flpe-ERT2 fusion protein
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.
Proper citation: RRID:Addgene_14756 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta S9A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7980435
Comments: S2A in the sequence does not affect function, used to introduce restriction enzyme site
Proper citation: RRID:Addgene_14754 Copy
Species: N. pharaonis
Genetic Insert: Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9500; Vector Backbone:FCK(1.3)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17375185
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_14750 Copy
Species: Homo sapiens
Genetic Insert: H-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Comments: GTPase deficient (dominant active) mutant: GAP insensitive, increased GDP/GTP exchange, and chronically GTP bound; transforming.
Proper citation: RRID:Addgene_14720 Copy
Vector Backbone Description: Backbone Size:6794; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD93.97, Ligation number NONE.
Proper citation: RRID:Addgene_1476 Copy
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