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Vector Backbone Description: Backbone Size:7721; Vector Backbone:pFRT; Vector Types:Mammalian Expression, Unspecified, pDEST GATEWAY; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30478324
Proper citation: RRID:Addgene_168207 Copy
Species: Catenaria anguillulae
Genetic Insert: YFP-CaCyclOp(A-2x)
Vector Backbone Description: Vector Backbone:pCFJ104; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168168 Copy
Species: Danio rerio
Genetic Insert: Csf3b
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24128862
Proper citation: RRID:Addgene_168202 Copy
Species: Synthetic
Genetic Insert: AncCAR_A
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:7284; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31799431
Proper citation: RRID:Addgene_168286 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST TnsD
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168166 Copy
Species: Blastocladiella emersonii
Genetic Insert: BeCNG1-YFP
Vector Backbone Description: Vector Backbone:pPD96.52; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168167 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST Tns proteins (TnsAB and TnsC)
Vector Backbone Description: Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168160 Copy
Species: Blastocladiella emersonii
Genetic Insert: YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
Vector Backbone Description: Backbone Marker:J. Rand; Vector Backbone:RM#348p; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168170 Copy
Species: Vesicular Stomatitis Virus
Genetic Insert: VSV-G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6150; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168223 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5520; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168220 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168188 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168189 Copy
Species: Homo sapiens
Genetic Insert: HA-nLag6-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168191 Copy
Species: Homo sapiens
Genetic Insert: flag-Chibby
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pCS3+FLT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16824 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Chibby
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of Drosophila Chibby, 0.3kb, was amplified by PCR and subcloned into the BamH1 and EcoR1 sites of pCS2P+.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16822 Copy
Species: Synthetic
Genetic Insert: Leucine Zipper + C-fragment of wtBlc
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33210733
Proper citation: RRID:Addgene_168256 Copy
Species: E. coli
Genetic Insert: C-fragment of wtBlc
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33210733
Proper citation: RRID:Addgene_168257 Copy
Genetic Insert: PBD GFP
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168252 Copy
Species: Drosophila melanogaster
Genetic Insert: armadillo
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2261639
Comments: Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.
T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.
Proper citation: RRID:Addgene_16829 Copy
Genetic Insert: RacFRET
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Comments: Reference for the RacFRET (Raichu-Rac1) element:
D. L. Graham, P. N. Lowe, and P. A. Chalk. A method to measure the interaction of Rac/Cdc42 with their binding partners using fluorescence resonance energy transfer between mutants of green fluorescent protein. Anal.Biochem. 296:208-217, 2001.
Proper citation: RRID:Addgene_168247 Copy
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