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Species: Homo sapiens
Genetic Insert: E2F7
Vector Backbone Description: Vector Backbone:pcDNA3.1V5hisTOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26957605
Proper citation: RRID:Addgene_73074 Copy
Species: Homo sapiens
Genetic Insert: E2F7
Vector Backbone Description: Vector Backbone:pcDNA3.1V5hisTOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26957605
Proper citation: RRID:Addgene_73078 Copy
Species: Homo sapiens
Genetic Insert: VEGFA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_73017 Copy
Species: Homo sapiens
Genetic Insert: INHBA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_73015 Copy
Species: Homo sapiens
Genetic Insert: kappa-myc-dL5-2XG4S-TM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5388; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650487
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
For more information about this plasmid, please see the associated publication (PMID: 25650487) as well as He et al. Nat Methods. 2016 Jan 25. doi: 10.1038/nmeth.3735. [Epub ahead of print] PMID: 26808669.
Proper citation: RRID:Addgene_73206 Copy
Species: Homo sapiens
Genetic Insert: FKBP(F36V)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21768107
Proper citation: RRID:Addgene_73180 Copy
Species: Homo sapiens
Genetic Insert: SNORD116-13
Vector Backbone Description: Backbone Marker:Zefeng Wang's lab; Backbone Size:4700; Vector Backbone:pZW1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25378317
Comments: Two snoRNA genes SNORD116-13 and SNORD116-14 from the Prader–Willi syndrome deletion region flanked by multiple cloning sites were inserted into the weak intron of Enhanced Green Fluoresence Protein (EGFP) such that only proper splicing leads to EGFP fluorescence.
Proper citation: RRID:Addgene_73174 Copy
Species: Homo sapiens
Genetic Insert: MRP1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73120 Copy
Species: Homo sapiens
Genetic Insert: MET7A
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73127 Copy
Species: Homo sapiens
Genetic Insert: PGRMC2
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73112 Copy
Species: Homo sapiens
Genetic Insert: CD58
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73113 Copy
Species: Homo sapiens
Genetic Insert: JAM1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73110 Copy
Species: Homo sapiens
Genetic Insert: ENDOD1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73114 Copy
Species: Homo sapiens
Genetic Insert: APMAP
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22080952
Proper citation: RRID:Addgene_73091 Copy
Species: Homo sapiens
Genetic Insert: human PUM1 Pumilio homology domain
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7477; Vector Backbone:pTYB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16954190
Proper citation: RRID:Addgene_73299 Copy
Species: Homo sapiens
Genetic Insert: human PUM1 Pumilio homology domain
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7477; Vector Backbone:pTYB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12202039
Proper citation: RRID:Addgene_73298 Copy
Species: Homo sapiens
Genetic Insert: 10XHis-GST-HRV-Her1-dL5
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5387; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25490520
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Proper citation: RRID:Addgene_73216 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5384; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25490520
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Proper citation: RRID:Addgene_73214 Copy
Species: Homo sapiens
Genetic Insert: 10XHis-GST-HRV-Her1-dL5-Her1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5387; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25490520
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
Note: Compared to the depositor's provided genbank file, Addgene's quality control sequencing finds one single nucleotide mismatch, G->A at bp 6867, which causes a G to R amino acid residue substitution. This discrepancy falls between the functional domains, and although it destroys a cloning site (BamHI) from the original clone construction, it should not otherwise alter plasmid function.
Proper citation: RRID:Addgene_73217 Copy
Species: Homo sapiens
Genetic Insert: FKBP1B
Vector Backbone Description: Backbone Marker:SGC, Addgene Plasmid #26103; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 for protein expression. See also 4C02: http://www.thesgc.org/structures/4C02
Proper citation: RRID:Addgene_73241 Copy
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