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Species: Saccharomyces cerevisiae
Genetic Insert: GALLpr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: This plasmid was made by Catherine Oikonomou in Dr. Cross's lab.
yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164702 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP1pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164703 Copy
Species: Homo sapiens
Genetic Insert: miR-451~451
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3114; Vector Backbone:pBI-CMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164667 Copy
Species: Homo sapiens
Genetic Insert: PRMT8
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_164700 Copy
Species: Homo sapiens
Genetic Insert: miR-125a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3114; Vector Backbone:pBI-CMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164668 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164707 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tetOpr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164705 Copy
Species: Homo sapiens
Genetic Insert: PRMT3
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25728001
Proper citation: RRID:Addgene_164695 Copy
Species: Homo sapiens
Genetic Insert: PRMT8
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_164699 Copy
Genetic Insert: GluN2A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25887710
Proper citation: RRID:Addgene_164724 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSAR-MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8755583
Comments: The pSAR-MT inducible
expression plasmid was constructed as follows. Two
oligonucleotides (5'-GATCTCGAGCTCCCTGCA-3' and 5'-GGGAGCTCGA-3') were annealed, and the resulting fragment was cloned into the BamHI and PstI sites of the scaffold attachment region (SAR)-containing plasmid p8. The resulting plasmid, pJM7, consisted of two SAR sequences
flanking a short polylinker containing restriction sites for Xhol and PstI. The mutant metallothionein (MT) promoter was amplified by PCR and cloned into pCEP4, linearized
with BglII and NotI, yielding pCEP-MT. A SalI-BamHI
fragment containing an intron from the globin gene was then
inserted into the XhoI and BamHI sites of pCEP-MT, yielding pCEP-MTi. Finally, the MT promoter/intron/poly(A) region was removed from pCEP-MTi by digestion with Sall and cloned into the XhoI site of pJM7, yielding pSAR-MT.
Proper citation: RRID:Addgene_16485 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-3
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164867 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-2
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164866 Copy
Genetic Insert: GluN2A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25887710
Proper citation: RRID:Addgene_164776 Copy
Species: Mus musculus
Genetic Insert: nAChR b2 E61C - IRES- GFP
Vector Backbone Description: Backbone Size:9424; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30176987
Proper citation: RRID:Addgene_164777 Copy
Species: Mus musculus
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796420
Proper citation: RRID:Addgene_16478 Copy
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619540
Proper citation: RRID:Addgene_164802 Copy
Genetic Insert: ERH CRISPRi gRNA1
Vector Backbone Description: Backbone Marker:Jonathan Weissman (Addgene plasmid # 60955); Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164800 Copy
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619540
Proper citation: RRID:Addgene_164804 Copy
Species: Homo sapiens
Genetic Insert: miR-144~451
Vector Backbone Description: Backbone Marker:Jonathan Weissman (Addgene plasmid # 60955); Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164793 Copy
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