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Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pRSFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28349302
Comments: Additional reference: Zhang, Y.; Du, W.X.; Fregevu, C.; Kothary, M.H.; Harden, L; McHugh, T.H. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. J. Agric Food Chem 2014, 62, 12695-700. PMID: 25437489
Proper citation: RRID:Addgene_87799 Copy
Species: Homo sapiens
Genetic Insert: SPAST
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20430936
Proper citation: RRID:Addgene_87715 Copy
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pRSFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28349302
Comments: Additional reference: Zhang, Y.; Du, W.X.; Fregevu, C.; Kothary, M.H.; Harden, L; McHugh, T.H. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. J. Agric Food Chem 2014, 62, 12695-700. PMID: 25437489
Proper citation: RRID:Addgene_87797 Copy
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pRSFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28349302
Comments: Additional reference: Zhang, Y.; Du, W.X.; Fregevu, C.; Kothary, M.H.; Harden, L; McHugh, T.H. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. J. Agric Food Chem 2014, 62, 12695-700. PMID: 25437489
Proper citation: RRID:Addgene_87798 Copy
Species: Homo sapiens
Genetic Insert: SPAST
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20430936
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICRI and ApaI sites removed myc tag. To detect spastin, use an anti-spastin antibody.
Proper citation: RRID:Addgene_87730 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1a, 17
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87900 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1a, 40a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87901 Copy
Species: Synthetic
Genetic Insert: sgGFP1, sgCas9
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:2600; Vector Backbone:pENTR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87908 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1, 5a, 40a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87889 Copy
Species: K. stuttgartiensis
Genetic Insert: Kuste2803
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26975050
Comments: protein sequence: MKKLVLINPHPMGNVGEENVSVLNQMPVNLGYLKVLTPGNWQVDIIDETQEYAIDENTGDITFGGADLVGITSVSYQADRAYKIATACRKRGIPVIMGGIHATSNPDEAAKYVDSVVIREGITLWSTIIDDFEKGRLRKRYDGGLTPMEIYCNLSPDRKFLKDKYKYRYSGIITTAGCPFACEFCSVPQFQGQKYRERPVDDILNEFESIKGQYRGLILTDENFYGHSKKSNERVRTLFKGMVERGIYQNWFGFTSLNIYKDDETLEYMAKSGCVGVLIGIESIEEESLKSMNKNVNLKISVENYYEAIANIRKHGLAVWGTMVFGNDADTSGTFERVADFILDSHIDIMTCGILCPFINTPLYHRLNRENRLFRTHFPEDWKYYTSHHLTYVLKKLTLDEMIEGFQYLYDRIFATEVLRKRFHRAKDELNNNMNAAMFAFRVNLDWQNVYQHLIQNLKDLHTSGDYEAALKCYVDTDKQGKGKKLASIEAAPLEHHHHHH-
Proper citation: RRID:Addgene_87922 Copy
Species: K. stuttgartiensis
Genetic Insert: Kuste3608
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26975050
Comments: protein sequence: MGSSHHHHHHSSGLVPRGSHMKKMMLINPHKPGRHGEESITVIVQMPLNLAYIKALTPGDWEFDVIDENIELAIDDNGELTFAPVDLVCITSVTYQSPRAYKIATACKRKGMTVIMGGIHASVMPEEASKYVDTVFIGEAEEVWPRVIKDFEAGKLKKVYDGGLPPLNLMKRVFPDREFLRKKYNYKFSSIVTTKGCPNYCDFCSVPTFQGRKFRERPYEDVLEELAATDYKGLMLAEDNFYGHGKRSNERARNLFKGMVERNLQKDWLGFTALNISQDKETLDYMAKSGCFGMLMGIESTNETVLEKMNKQVNLKLGTESYYDCIQKIHDAGLVTWGSVVFGADGDGKDSFKRMTDFILENNIDILTFGINCPFPKTQLYKRLDSEKRIFRKNYPDDWEYYDTAHVVHRLVDMTLEDFIEGMQYVYDHIYAGDNLRKRFRNSIKTTNNPRNSMFAFRVGSDWQQVFDQVLENLRLLYDSGDYYKDYYKSNSVSVSKKPLVESITT-
Proper citation: RRID:Addgene_87923 Copy
Species: K. stuttgartiensis
Genetic Insert: Kuste2803
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26975050
Comments: protein sequence: MGSSHHHHHHSSGLVPRGSHMKKLVLINPHPMGNVGEENVSVLNQMPVNLGYLKVLTPGNWQVDIIDETQEYAIDENTGDITFGGADLVGITSVSYQADRAYKIATACRKRGIPVIMGGIHATSNPDEAAKYVDSVVIREGITLWSTIIDDFEKGRLRKRYDGGLTPMEIYCNLSPDRKFLKDKYKYRYSGIITTAGCPFACEFCSVPQFQGQKYRERPVDDILNEFESIKGQYRGLILTDENFYGHSKKSNERVRTLFKGMVERGIYQNWFGFTSLNIYKDDETLEYMAKSGCVGVLIGIESIEEESLKSMNKNVNLKISVENYYEAIANIRKHGLAVWGTMVFGNDADTSGTFERVADFILDSHIDIMTCGILCPFINTPLYHRLNRENRLFRTHFPEDWKYYTSHHLTYVLKKLTLDEMIEGFQYLYDRIFATEVLRKRFHRAKDELNNNMNAAMFAFRVNLDWQNVYQHLIQNLKDLHTSGDYEAALKCYVDTDKQGKGKKLASIEAAP-
Proper citation: RRID:Addgene_87921 Copy
Species: K. stuttgartiensis
Genetic Insert: Kuste3608
Vector Backbone Description: Vector Backbone:pET28a-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26975050
Comments: protein sequence: MGSSHHHHHHSSGKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTSSGLVPRGSHMKKMMLINPHKPGRHGEESITVIVQMPLNLAYIKALTPGDWEFDVIDENIELAIDDNGELTFAPVDLVCITSVTYQSPRAYKIATACKRKGMTVIMGGIHASVMPEEASKYVDTVFIGEAEEVWPRVIKDFEAGKLKKVYDGGLPPLNLMKRVFPDREFLRKKYNYKFSSIVTTKGCPNYCDFCSVPTFQGRKFRERPYEDVLEELAATDYKGLMLAEDNFYGHGKRSNERARNLFKGMVERNLQKDWLGFTALNISQDKETLDYMAKSGCFGMLMGIESTNETVLEKMNKQVNLKLGTESYYDCIQKIHDAGLVTWGSVVFGADGDGKDSFKRMTDFILENNIDILTFGINCPFPKTQLYKRLDSEKRIFRKNYPDDWEYYDTAHVVHRLVDMTLEDFIEGMQYVYDHIYAGDNLRKRFRNSIKTTNNPRNSMFAFRVGSDWQQVFDQVLENLRLLYDSGDYYKDYYKSNSVSVSKKPLVESITT-
Proper citation: RRID:Addgene_87924 Copy
Species: Homo sapiens
Genetic Insert: MKK6
Vector Backbone Description: Vector Backbone:pET28 NKI LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28242696
Proper citation: RRID:Addgene_87882 Copy
Species: Homo sapiens
Genetic Insert: MKK4
Vector Backbone Description: Vector Backbone:pET28 NKI LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_87880 Copy
Species: Homo sapiens
Genetic Insert: MKK3
Vector Backbone Description: Vector Backbone:pET28 NKI LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_87878 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1a, 5a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87898 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1a, 17, 40a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87899 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1a, 5a, 17
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87896 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1a, 5a, 40a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87897 Copy
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