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Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD94.81, Ligation number NONE.
Proper citation: RRID:Addgene_1477 Copy
Species: Homo sapiens
Genetic Insert: DGCR8 shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: TGGGGTGAGA GTGCTGATAA TTCAAGAGAT TATCAGCACT CTCACCCCTT TTTTC
Proper citation: RRID:Addgene_14769 Copy
Species: Homo sapiens
Genetic Insert: Drosha shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: TGAAGCTCGA TGAAGATTTA TTCAAGAGAT AAATCTTCAT CGAGCTTCTT TTTTC
Proper citation: RRID:Addgene_14766 Copy
Species: Homo sapiens
Genetic Insert: Drosha shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: TGAAGCTCTT TGGTGAATAA TTCAAGAGAT TATTCACCAA AGAGCTTCTT TTTTC
Proper citation: RRID:Addgene_14767 Copy
Species: Mus musculus
Genetic Insert: let-7g
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6500; Vector Backbone:MSCV-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: miRNA of the following mature sequence, with approximately 500 basepairs of flanking sequence, were cloned into MSCV-neo: let-7g: 5’-UGAGG UAGUA GUUUG UACAG U-3’.
Proper citation: RRID:Addgene_14784 Copy
Species: Mus musculus
Genetic Insert: Hmga2 m7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17322030
Comments: The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).
Full sequence of wild-type plasmid available at Addgene plasmid #14789.
Proper citation: RRID:Addgene_14792 Copy
Vector Backbone Description: Backbone Size:8846; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD95.16, Ligation number NONE.
Proper citation: RRID:Addgene_1483 Copy
Species: Mus musculus
Genetic Insert: Smad7
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe puro 3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10791980
Comments: See Author's map for more information.
Proper citation: RRID:Addgene_14836 Copy
Species: Synthetic
Genetic Insert: PuroTK
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Comments: Plate note: Plasmid contains a single G nucleotide deletion in the attL1 sequence. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141005 Copy
Species: Ralstonia eutropha H16
Genetic Insert: phaCAB
Vector Backbone Description: Backbone Size:2883; Vector Backbone:pHB-2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32339760
Proper citation: RRID:Addgene_140957 Copy
Species: Synthetic
Genetic Insert: HygTK
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141006 Copy
Species: Escherichia coli
Genetic Insert: Neomycin resistance gene
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141007 Copy
Species: Synthetic
Genetic Insert: TetR-sfGFP expressed with T3 promoter
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30487584
Proper citation: RRID:Addgene_140871 Copy
Species: Discosoma
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pMV361-strep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:32829077
Comments: tdTomato: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry
(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037.
Proper citation: RRID:Addgene_140994 Copy
Species: simian virus
Genetic Insert: Large T antigen
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12781366
Proper citation: RRID:Addgene_14088 Copy
Species: Mus musculus
Genetic Insert: p19 ARF shRNA -2
Vector Backbone Description: Backbone Marker:Available from Addgene (#11795); Backbone Size:8000; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12853964
Comments: p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.
Proper citation: RRID:Addgene_14091 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:pCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141112 Copy
Species: Homo sapiens
Genetic Insert: GCN5
Vector Backbone Description: Backbone Size:25000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_14106 Copy
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG FlpE to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Please visit https://www.biorxiv.org/content/10.1101/571323v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141111 Copy
Genetic Insert: zCas9(H840A)-OsM-MLV-RT
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9(H840A)-OsM-MLV-RT; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32222487
Comments: Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.
Proper citation: RRID:Addgene_141080 Copy
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