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Species: Mus musculus
Genetic Insert: GDNF family receptor alpha-2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR2.1; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20064391
Comments: This plasmid can be used to generate an in situ hybridization antisense probe for mouse Gfr-alpha2. Acc65I or SpeI can be used to linearize the plasmid followed by SP6 to transcribe the ~1.5kb probe.
Proper citation: RRID:Addgene_119899 Copy
Species: Mus musculus
Genetic Insert: neurturin probe
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25838128
Comments: This plasmid can be used to generate an in situ hybridization antisense probe for mouse NRTN, using T7 to transcribe the ~1kb probe.
Proper citation: RRID:Addgene_119896 Copy
Species: Mus musculus
Genetic Insert: glial cell line derived neurotrophic factor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25838128
Comments: This plasmid can be used to generate an in situ hybridization antisense probe for mouse GDNF, using SP6 to transcribe the ~1kb probe.
Proper citation: RRID:Addgene_119897 Copy
Species: Mus musculus
Genetic Insert: c-jun promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5000; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9448004
Comments: Plasmids pJC6GL3, pJSXGL3, pJTXGL3, and pJSTXGL3 contain positions -225 to +150 of the murine c-jun promoter. pJSXGL3, pJTXGL3, and pJSTXGL3 contain mutations at the MEF2 site, ATF (AP1-like) site, and both sites, respectively.
Proper citation: RRID:Addgene_11980 Copy
Species: Mus musculus
Genetic Insert: c-jun promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5000; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9448004
Comments: Plasmids pJC6GL3, pJSXGL3, pJTXGL3, and pJSTXGL3 contain positions -225 to +150 of the murine c-jun promoter. pJSXGL3, pJTXGL3, and pJSTXGL3 contain mutations at the MEF2 site, ATF (AP1-like) site, and both sites, respectively.
Proper citation: RRID:Addgene_11982 Copy
Species: Mus musculus
Genetic Insert: c-jun promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5000; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9448004
Comments: Plasmids pJC6GL3, pJSXGL3, pJTXGL3, and pJSTXGL3 contain positions -225 to +150 of the murine c-jun promoter. pJSXGL3, pJTXGL3, and pJSTXGL3 contain mutations at the MEF2 site, ATF (AP1-like) site, and both sites, respectively.
Proper citation: RRID:Addgene_11981 Copy
Species: Mus musculus
Genetic Insert: Type I procollagen α2 chain
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30287488
Comments: These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete.
We recommend following these guidelines when using the plasmids
1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs.
2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed).
3) Optimization of transfection efficiency is needed for all cell types.
4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis.
5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction.
6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death.
Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]).
Proper citation: RRID:Addgene_119836 Copy
Species: Mus musculus
Genetic Insert: kinesin family member 5B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3954; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31100061
Proper citation: RRID:Addgene_120170 Copy
Species: Mus musculus
Genetic Insert: Ccdc167
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120242 Copy
Species: Mus musculus
Genetic Insert: Bet1
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120243 Copy
Species: Mus musculus
Genetic Insert: Emerin
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31142202
Proper citation: RRID:Addgene_120246 Copy
Species: Mus musculus
Genetic Insert: Tmx4
Vector Backbone Description: Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31142202
Proper citation: RRID:Addgene_120236 Copy
Species: Mus musculus
Genetic Insert: CAG:gfp:P2A:myc_NRXN
Vector Backbone Description: Vector Backbone:paavCAG-Jx; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120176 Copy
Species: Mus musculus
Genetic Insert: Ovol1
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30639212
Proper citation: RRID:Addgene_120360 Copy
Species: Mus musculus
Genetic Insert: Ovol1
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30639212
Proper citation: RRID:Addgene_120359 Copy
Species: Mus musculus
Genetic Insert: OKMS cassette
Vector Backbone Description: Vector Backbone:PB-TAC-KRAB-dCas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30639212
Comments: Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120356 Copy
Species: Mus musculus
Genetic Insert: OK+9MS cassette
Vector Backbone Description: Vector Backbone:PB-TAC-KRAB-dCas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30639212
Comments: Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120357 Copy
Species: Mus musculus
Genetic Insert: Diras2 shRNA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pAAV; Vector Types:AAV, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30623719
Comments: The original backbone was published in Candemir et al. Eur Neuropsychopharmacol. 2016 (PMID: 26861996)
Proper citation: RRID:Addgene_120394 Copy
Species: Mus musculus
Genetic Insert: Lin28
Vector Backbone Description: Vector Backbone:pJET1.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120535 Copy
Species: Mus musculus
Genetic Insert: Lin28
Vector Backbone Description: Vector Backbone:pJET1.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120536 Copy
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