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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGEX-2T-IQGAP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30107 | IQGAP1 | Homo sapiens | Ampicillin | PMID:9867866 | Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:24 | 1 | ||
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pGFPHYG2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30173 | Mycobacterium Strong Promoter (MSP) | M. marinum | Hygromycin | PMID:16714540 | This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. | Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin | GFPmut3 allele has improved brightness. | 2026-08-15 01:13:25 | 5 |
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pMXs-GLIS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30166 | GLIS1 | Homo sapiens | Ampicillin | PMID:21654807 | pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation. IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274 | Backbone Marker:Shinya Yamanaka; Backbone Size:4700; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:23 | 4 | |
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pLKO.1 RagD shRNA #1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30321 | RagD shRNA | Homo sapiens | Ampicillin | PMID:18497260 | shRNA directed against RagD. Target sequence: 5'-CGGCAAGTCGTCTATTCAGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute. | Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:27 | 1 | |
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w+attB Resource Report Resource Website 10+ mentions |
RRID:Addgene_30326 | phiC31 attB, hsp70::w | Drosophila melanogaster | Ampicillin | w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock. | Backbone Size:2881; Vector Backbone:pUC; Vector Types:Drosophila transgenesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:24 | 12 | ||
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AAV-Flex-rev-oChIEF-tdTomato Resource Report Resource Website 10+ mentions |
RRID:Addgene_30541 | oChIEF-tdtomato | Chlamydomonas reinhardtii | Ampicillin | PMID:21878344 | Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function. To minimize recombination, propagate this plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. For more information on growth conditions, please see www.addgene.org/18917 as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence. Please see Reviews in the right column for an image of Addgene's digest with these enzymes. | Backbone Marker:Addgene plasmid 18917; Backbone Size:5079; Vector Backbone:AAV-FLEX-rev-ChR2-tdtomato; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:29 | 11 | |
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hrGFP-BATS Resource Report Resource Website 1+ mentions |
RRID:Addgene_30496 | Barkor | Homo sapiens | Ampicillin | PMID:21518905 | Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 413-492 aa | 2026-08-15 01:13:28 | 1 | |
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pET24c(+)-mtEFTu Resource Report Resource Website 1+ mentions |
RRID:Addgene_30535 | Mature bovine mitochondrial elongation factor Tu | Bos taurus | Kanamycin | PMID:8547323 | Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Mature form amino acids 44-452. Mito import sequence not included | 2026-08-15 01:13:29 | 1 | |
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pLV.SP146.gp91.GP91.cHS4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30480 | SP146/gp91/GP91/cHS4 | Ampicillin | PMID:21544095 | From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France), the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive site and was provided by Patrick Salmon (Department of Neuroscience, Faculty of Medicine, University of Geneva, Switzerland). | Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:28 | 3 | ||
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pCMVβ-p300-myc Resource Report Resource Website 10+ mentions |
RRID:Addgene_30489 | p300 | Homo sapiens | Ampicillin | Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:25 | 25 | |||
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pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB Resource Report Resource Website 1+ mentions |
RRID:Addgene_30505 | beta-glucuronidase | Escherichia coli | Ampicillin | PMID:22020504 | In our experience, plasmid yields are highest when cells are harvest at late log stage. Note that this plasmid also confers resistance to Spectinomycin, but Addgene provides it in an Amp stab. | Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:29 | 2 | |
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pBOB-synP-HT Resource Report Resource Website 1+ mentions |
RRID:Addgene_30456 | histone GFP | Ampicillin | Backbone Marker:Addgene plasmid 12337; Backbone Size:8000; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:25 | 6 | ||||
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pLR-NI Resource Report Resource Website 1+ mentions |
RRID:Addgene_31006 | last NI repeat | Xanthamonas oryzae | Spectinomycin | PMID:21493687 | Backbone Size:2460; Vector Backbone:pCS468; Vector Types:; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:31 | 2 | ||
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pLR-NG Resource Report Resource Website 1+ mentions |
RRID:Addgene_30995 | last NG repeat | Xanthamonas oryzae | Spectinomycin | PMID:21493687 | Backbone Size:2460; Vector Backbone:pCS468; Vector Types:; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:27 | 2 | ||
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pNG3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30987 | repeat NG3 | Xanthamonas oryzae | Tetracycline | PMID:21493687 | Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline | 2026-08-15 01:13:30 | 1 | ||
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pNG2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_30986 | repeat NG2 | Xanthamonas oryzae | Tetracycline | PMID:21493687 | Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline | 2026-08-15 01:13:27 | 1 | ||
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pFUS_B4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31021 | LacZ + BsaI restriction sites | Spectinomycin | PMID:21493687 | Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:31 | 1 | |||
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pFUS_B1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31018 | LacZ + BsaI restriction sites | Spectinomycin | PMID:21493687 | Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin | 2026-08-15 01:13:31 | 1 | |||
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pCMV:GFP(FRT)lacZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_31124 | GFP(FRT)lacZ | Ampicillin | PMID:11376165 | FLP reporter: from green protein fluorescence to lacZ | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, FLP/FRT; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:29 | 1 | ||
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pLenti6-MK1-EHMT2-V5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_31113 | euchromatic histone-lysine N-methyltransferase 2 | Homo sapiens | Ampicillin | Spektor TM, Rice JC. Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14808-13. Epub 2009 Aug 18. | Backbone Marker:Invitrogen; Backbone Size:6963; Vector Backbone:pLenti6/V5-D-TOPO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Stop codon mutated to Ala | 2026-08-15 01:13:32 | 4 |
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