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http://www.addgene.org/30107

Species: Homo sapiens
Genetic Insert: IQGAP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9867866

Proper citation: RRID:Addgene_30107 Copy   

•   Source: Addgene

http://www.addgene.org/30173

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:16714540
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.

Proper citation: RRID:Addgene_30173 Copy   

•   Source: Addgene

http://www.addgene.org/30166

Species: Homo sapiens
Genetic Insert: GLIS1
Vector Backbone Description: Backbone Marker:Shinya Yamanaka; Backbone Size:4700; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21654807
Comments: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation. IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274

Proper citation: RRID:Addgene_30166 Copy   

•   Source: Addgene

http://www.addgene.org/30321

Species: Homo sapiens
Genetic Insert: RagD shRNA
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18497260
Comments: shRNA directed against RagD. Target sequence: 5'-CGGCAAGTCGTCTATTCAGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute.

Proper citation: RRID:Addgene_30321 Copy   

•   Source: Addgene

http://www.addgene.org/30326

Species: Drosophila melanogaster
Genetic Insert: phiC31 attB, hsp70::w
Vector Backbone Description: Backbone Size:2881; Vector Backbone:pUC; Vector Types:Drosophila transgenesis; Bacterial Resistance:Ampicillin
Comments: w[+] is hsp70 basal promoter; gives full w+ (red eyes), without heat shock.

Proper citation: RRID:Addgene_30326 Copy   

•   Source: Addgene

http://www.addgene.org/30541

Species: Chlamydomonas reinhardtii
Genetic Insert: oChIEF-tdtomato
Vector Backbone Description: Backbone Marker:Addgene plasmid 18917; Backbone Size:5079; Vector Backbone:AAV-FLEX-rev-ChR2-tdtomato; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21878344
Comments: Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function. To minimize recombination, propagate this plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. For more information on growth conditions, please see www.addgene.org/18917 as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence. Please see Reviews in the right column for an image of Addgene's digest with these enzymes.

Proper citation: RRID:Addgene_30541 Copy   

•   Source: Addgene

http://www.addgene.org/30496

Species: Homo sapiens
Genetic Insert: Barkor
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:4300; Vector Backbone:phrGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21518905

Proper citation: RRID:Addgene_30496 Copy   

•   Source: Addgene

http://www.addgene.org/30535

Species: Bos taurus
Genetic Insert: Mature bovine mitochondrial elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8547323

Proper citation: RRID:Addgene_30535 Copy   

•   Source: Addgene

http://www.addgene.org/30480

Genetic Insert: SP146/gp91/GP91/cHS4
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21544095
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France), the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive site and was provided by Patrick Salmon (Department of Neuroscience, Faculty of Medicine, University of Geneva, Switzerland).

Proper citation: RRID:Addgene_30480 Copy   

•   Source: Addgene

http://www.addgene.org/30489

Species: Homo sapiens
Genetic Insert: p300
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMVb; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_30489 Copy   

•   Source: Addgene

http://www.addgene.org/30505

Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22020504
Comments: In our experience, plasmid yields are highest when cells are harvest at late log stage. Note that this plasmid also confers resistance to Spectinomycin, but Addgene provides it in an Amp stab.

Proper citation: RRID:Addgene_30505 Copy   

•   Source: Addgene

http://www.addgene.org/30456

Genetic Insert: histone GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 12337; Backbone Size:8000; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_30456 Copy   

•   Source: Addgene

http://www.addgene.org/31006

Species: Xanthamonas oryzae
Genetic Insert: last NI repeat
Vector Backbone Description: Backbone Size:2460; Vector Backbone:pCS468; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_31006 Copy   

•   Source: Addgene

http://www.addgene.org/30995

Species: Xanthamonas oryzae
Genetic Insert: last NG repeat
Vector Backbone Description: Backbone Size:2460; Vector Backbone:pCS468; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_30995 Copy   

•   Source: Addgene

http://www.addgene.org/30987

Species: Xanthamonas oryzae
Genetic Insert: repeat NG3
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_30987 Copy   

•   Source: Addgene

http://www.addgene.org/30986

Species: Xanthamonas oryzae
Genetic Insert: repeat NG2
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_30986 Copy   

•   Source: Addgene

http://www.addgene.org/31021

Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_31021 Copy   

•   Source: Addgene

http://www.addgene.org/31018

Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687

Proper citation: RRID:Addgene_31018 Copy   

•   Source: Addgene

http://www.addgene.org/31124

Genetic Insert: GFP(FRT)lacZ
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11376165
Comments: FLP reporter: from green protein fluorescence to lacZ

Proper citation: RRID:Addgene_31124 Copy   

•   Source: Addgene

http://www.addgene.org/31113

Species: Homo sapiens
Genetic Insert: euchromatic histone-lysine N-methyltransferase 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6963; Vector Backbone:pLenti6/V5-D-TOPO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Spektor TM, Rice JC. Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14808-13. Epub 2009 Aug 18.

Proper citation: RRID:Addgene_31113 Copy   

•   Source: Addgene