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Plasmids are provided by Addgene and DGRC.

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On page 33 showing 641 ~ 660 out of 1,658 results
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  • RRID:Addgene_58550

http://www.addgene.org/58550

Vector Backbone Description: Backbone Size:8098; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin

Proper citation: RRID:Addgene_58550 Copy   


  • RRID:Addgene_58552

http://www.addgene.org/58552

Vector Backbone Description: Backbone Size:7084; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin

Proper citation: RRID:Addgene_58552 Copy   


  • RRID:Addgene_59205

    This resource has 1+ mentions.

http://www.addgene.org/59205

Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein. The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.

Proper citation: RRID:Addgene_59205 Copy   


  • RRID:Addgene_59204

http://www.addgene.org/59204

Vector Backbone Description: Backbone Marker:van Steensal lab, Addgene plasmid 59201; Backbone Size:7660; Vector Backbone:pIND(V5)EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: Note that this vector can not be used to express Dam only.

Proper citation: RRID:Addgene_59204 Copy   


  • RRID:Addgene_59382

    This resource has 1+ mentions.

http://www.addgene.org/59382

Species: Synthetic
Genetic Insert: TT-ISceI-cat
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.

Proper citation: RRID:Addgene_59382 Copy   


  • RRID:Addgene_59385

http://www.addgene.org/59385

Species: Synthetic
Genetic Insert: TT-ISceI-cat2
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.

Proper citation: RRID:Addgene_59385 Copy   


  • RRID:Addgene_71188

http://www.addgene.org/71188

Vector Backbone Description: Vector Backbone:pSGE_240_pAGW_V5; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26550828

Proper citation: RRID:Addgene_71188 Copy   


  • RRID:Addgene_71279

http://www.addgene.org/71279

Vector Backbone Description: Vector Backbone:pSW_1; Vector Types:Gateway vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26550828

Proper citation: RRID:Addgene_71279 Copy   


  • RRID:Addgene_71499

    This resource has 1+ mentions.

http://www.addgene.org/71499

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.

Proper citation: RRID:Addgene_71499 Copy   


  • RRID:Addgene_71503

http://www.addgene.org/71503

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.

Proper citation: RRID:Addgene_71503 Copy   


  • RRID:Addgene_71504

    This resource has 1+ mentions.

http://www.addgene.org/71504

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.

Proper citation: RRID:Addgene_71504 Copy   


  • RRID:Addgene_71502

http://www.addgene.org/71502

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.

Proper citation: RRID:Addgene_71502 Copy   


  • RRID:Addgene_71507

http://www.addgene.org/71507

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091

Proper citation: RRID:Addgene_71507 Copy   


  • RRID:Addgene_71508

http://www.addgene.org/71508

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091

Proper citation: RRID:Addgene_71508 Copy   


  • RRID:Addgene_71510

    This resource has 1+ mentions.

http://www.addgene.org/71510

Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017). Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII

Proper citation: RRID:Addgene_71510 Copy   


  • RRID:Addgene_54367

http://www.addgene.org/54367

Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites

Proper citation: RRID:Addgene_54367 Copy   


  • RRID:Addgene_54366

http://www.addgene.org/54366

Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites

Proper citation: RRID:Addgene_54366 Copy   


  • RRID:Addgene_54402

http://www.addgene.org/54402

Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: ihpRNA silencing vector (cloning only)

Proper citation: RRID:Addgene_54402 Copy   


  • RRID:Addgene_54373

http://www.addgene.org/54373

Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites

Proper citation: RRID:Addgene_54373 Copy   


  • RRID:Addgene_91797

    This resource has 10+ mentions.

http://www.addgene.org/91797

Vector Backbone Description: Backbone Marker:David Root Lab (addgene #41390); Backbone Size:9621; Vector Backbone:pLEX_305; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29581585

Proper citation: RRID:Addgene_91797 Copy   



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