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Species: Other
Genetic Insert: ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/COGhnD32 . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113648 Copy
Species: Other
Genetic Insert: ndmB
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/2C3Oh1ew . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113647 Copy
Species: Other
Genetic Insert: ndmA
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/4vRm4arA . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113646 Copy
Species: Other
Genetic Insert: mKaxxte2.5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30804405
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
Proper citation: RRID:Addgene_113630 Copy
Species: Other
Genetic Insert: ndmB and ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/kNCaeGXO . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113651 Copy
Species: Other
Genetic Insert: promoter T5 and sgRNA
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113655 Copy
Species: Other
Genetic Insert: promoter J23119 and sgRNA
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113654 Copy
Species: Other
Genetic Insert: ndmA and ndmB and ndmC
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/xeoN8t6c . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113652 Copy
Species: Other
Genetic Insert: TDK gene promoter and dCas9
Vector Backbone Description: Backbone Size:2906; Vector Backbone:pYTK095; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30703321
Comments: Note: This plasmid contains a L64H mutation in Cas9. This mutation is not known to affect plasmid function.
Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113657 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113750 Copy
Species: Other
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2-mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113753 Copy
Species: Other
Genetic Insert: mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mRuby2 at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113752 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP-mEGFP at your locus of interest.
Proper citation: RRID:Addgene_113751 Copy
Species: Other
Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3048; Vector Backbone:pGEM-T easy; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.
Proper citation: RRID:Addgene_113758 Copy
Species: Other
Genetic Insert: mRuby2-mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mRuby2-mRuby2-mRuby2 at your locus of interest.
Proper citation: RRID:Addgene_113757 Copy
Species: Other
Genetic Insert: mRuby2-mRuby2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert C-terminal mRuby2-mRuby2 at your locus of interest.
Proper citation: RRID:Addgene_113756 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR221-P5P4; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113741 Copy
Species: Other
Genetic Insert: mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113746 Copy
Species: Other
Genetic Insert: mEGFP-mEGFP-mEGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to insert N-terminal mEGFP-mEGFP-mEGFP at your locus of interest (stop codon removed).
Proper citation: RRID:Addgene_113748 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221-P1P2; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113735 Copy
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