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Species: Synthetic
Genetic Insert: F2620-rbs33-hrpR-ter
Vector Backbone Description: Backbone Marker:BioBrick registry; Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22009040
Proper citation: RRID:Addgene_61436 Copy
Species: Synthetic
Genetic Insert: hrpL-rbs30-gfp-ter
Vector Backbone Description: Backbone Marker:BioBrick registry; Backbone Size:3547; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22009040
Proper citation: RRID:Addgene_61437 Copy
Species: Synthetic
Genetic Insert: HrpL-rbs32-CI-LVA-t-plam-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick registry; Backbone Size:3547; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22009040
Proper citation: RRID:Addgene_61438 Copy
Species: Synthetic
Genetic Insert: HrpL-rbs34-CI-LVA-t-plam-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick registry; Backbone Size:3547; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22009040
Proper citation: RRID:Addgene_61439 Copy
Species: Synthetic
Genetic Insert: chromatin insulator flanked TRE-LSL-tdTomato
Vector Backbone Description: Backbone Size:2682; Vector Backbone:pUC57; Vector Types:Recombinase-mediated cassette exchange using Flp recombinase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Proper citation: RRID:Addgene_61576 Copy
Species: Synthetic
Genetic Insert: ntdTomato
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25643149
Comments: Please note that Addgene NGS results identified as stop codon after the second EGFP ORF. This stop codon was present in the original, published version of the plasmid, such that the insert is n2GFP rather than n3GFP. The plasmid functions as described in the associated publication. Co-expression with the helper plasmid pSOUP is suggested for expression in Agrobacterium.
The diagnostic digest indicates this plasmid may be bigger than the assembled sequence suggests.
Proper citation: RRID:Addgene_61628 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a C terminal Myc tag in frame with pHoran.
Proper citation: RRID:Addgene_61557 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Proper citation: RRID:Addgene_61558 Copy
Species: Synthetic
Genetic Insert: pHuji
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a N-terminal HA tag in frame with pHuji.
Proper citation: RRID:Addgene_61556 Copy
Species: Synthetic
Genetic Insert: HIV-1 integrase F185H/C280S
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8631811
Proper citation: RRID:Addgene_61672 Copy
Species: Synthetic
Genetic Insert: HIV-1 integrase W235F
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16482214
Proper citation: RRID:Addgene_61681 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61736 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61737 Copy
Species: Synthetic
Genetic Insert: scTALEN-ABL1-R
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336919
Proper citation: RRID:Addgene_61695 Copy
Species: Synthetic
Genetic Insert: Hygromycin
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCHYG. For more information, see: Motteram et al, 2009. Molecular Plant-Microbe Interactions, 22, 790-799. https://www.ncbi.nlm.nih.gov/pubmed/19522561.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and hygromycin sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication (Sidhu et al., 2015) for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Proper citation: RRID:Addgene_61765 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62147 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62148 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62149 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62143 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62140 Copy
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