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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4787; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35452 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4788; Vector Backbone:pRS31x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35451 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35566 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35565 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35564 Copy
Species: Danio rerio
Genetic Insert: Kdrl-5p-FokI-EL
Vector Backbone Description: Backbone Size:5026; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602
Proper citation: RRID:Addgene_35684 Copy
Species: Homo sapiens
Genetic Insert: bad
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Comments: A G21C point mutation is present in the sequence. The depositing lab notes that this should not alter the function of the plasmid.
Proper citation: RRID:Addgene_35569 Copy
Species: Homo sapiens
Genetic Insert: bad
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35568 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35562 Copy
Species: Homo sapiens
Genetic Insert: nsa-mir-224
Vector Backbone Description: Backbone Marker:provided by PESCHLE C. (PMID:163300772) - CONDORELLI G (PMID: 12692591); Backbone Size:8600; Vector Backbone:pRRL-CMV-PGK-GFP-WPRE (TWEEN); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Note that though there is GFP in this plasmid, it is not fused to the miR-224. There is a PGK promoter between the miR and the GFP.
Proper citation: RRID:Addgene_35683 Copy
Species: Homo sapiens
Genetic Insert: ERbeta
Vector Backbone Description: Vector Backbone:pSUPER puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19106194
Proper citation: RRID:Addgene_35561 Copy
Species: Synthetic
Genetic Insert: miR-122Zip
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5124; Vector Backbone:pRNA-U6.1/Neo-siFluc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35671 Copy
Species: Synthetic
Genetic Insert: let-7Zip
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5124; Vector Backbone:pRNA-U6.1/Neo-siFluc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Comments: Please note that there is a 1bp deletion in Addgene's quality control sequence when compared to depositor's reference sequence. This deletion will not affect plasmid function.
Proper citation: RRID:Addgene_35670 Copy
Species: Synthetic
Genetic Insert: U6 miR-122sponge
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5124; Vector Backbone:pRNA-U6.1/Neo-siFluc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35665 Copy
Species: Synthetic
Genetic Insert: U6 let-7sponge
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5124; Vector Backbone:pRNA-U6.1/Neo-siFluc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35664 Copy
Species: Synthetic
Genetic Insert: 7 mutant miR-122 target sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pgl3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35663 Copy
Species: Synthetic
Genetic Insert: TuD miR-122
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5124; Vector Backbone:pRNA-U6.1/Neo-siFluc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35669 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:InVitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22412018
Proper citation: RRID:Addgene_35700 Copy
Species: Synthetic
Genetic Insert: TuD let-7
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pAAVscCBPIGpluc7xlet-7BT; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35651 Copy
Species: Synthetic
Genetic Insert: miR-122 sponge
Vector Backbone Description: Backbone Size:5915; Vector Backbone:pAAV TBG FFluc; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Comments: Please note that there is a 4bp deletion in Addgene's quality control sequence when compared to depositor's reference sequence. This deletion will not affect plasmid function.
Proper citation: RRID:Addgene_35657 Copy
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