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Species: Other
Genetic Insert: D6GRK4(Cas9 coding gene from Filifactor alocis (strain ATCC 35896 / D40 B5))
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29529247
Proper citation: RRID:Addgene_103097 Copy
Species: Other
Genetic Insert: Q73QW6(Cas9 coding gene from Streptococcus mutans serotype c (strain ATCC 700610 / UA159))
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29529247
Proper citation: RRID:Addgene_103130 Copy
Species: Other
Genetic Insert: Tetracycline repressor
Vector Backbone Description: Backbone Size:7600; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33963794
Proper citation: RRID:Addgene_160235 Copy
Species: Other
Genetic Insert: Cym Operator (2x)
Vector Backbone Description: Backbone Size:7400; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33963794
Proper citation: RRID:Addgene_160242 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32571767
Comments: Plasmid may be particularly prone to recombination; please screen multiple colonies before selecting a clone to perform downstream experiments
SFS in paper should be FSF
Proper citation: RRID:Addgene_160551 Copy
Species: Other
Genetic Insert: PLY-WT-no charge
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pTricHisA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199689
Comments: ILY gene is codon optimized, and all native Cys removed, before additional mutations were added.
Proper citation: RRID:Addgene_160539 Copy
Species: Other
Genetic Insert: ER TurboID
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199915
Proper citation: RRID:Addgene_160857 Copy
Species: Other
Genetic Insert: Bovine Rhodopsin promoter driving DsRed fluorescent protein
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3200; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23818646
Proper citation: RRID:Addgene_173490 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173699 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173696 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173697 Copy
Species: Other
Genetic Insert: HK022 Integrase
Vector Backbone Description: Backbone Marker:Haldimann and Wanner 2001; Vector Backbone:pAH69; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Proper citation: RRID:Addgene_173782 Copy
Species: Other
Genetic Insert: Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Vector Backbone Description: Vector Backbone:pET-23b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26092728
Comments: Bacillus thuringiensis PI-PLC (UniProt P08954) cleaves GPI-anchored proteins off the surface of cells and can be used instead of the 97% identical, commercially available B. cereus enzyme (UniProt P14262).
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173808 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pE-FLP; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Comments: Depositor confirms the S102Y and S279Y in Cre does not affect plasmid function.
The pE_Cre_lambdaCos plasmid can be packaged in lambda phages using the CY2120 cosmid packaging strain [Cronan 2013]. Lambda infection provides a rapid and efficient way to deliver the pE_Cre_lambdaCos plasmid.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_173785 Copy
Species: Other
Genetic Insert: Staphylococcus aureus plc
Vector Backbone Description: Vector Backbone:pET-21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22390775
Comments: See UniProt ID P45723 for the mature, untagged sequence.
Also see PubMed 30148347 (bacterial PI-PLC review).
Proper citation: RRID:Addgene_173810 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173841 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173842 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173844 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/52961/; Vector Backbone:LentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173840 Copy
Species: Other
Genetic Insert: MiCy with mitochondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174177 Copy
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