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Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP4
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP4 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154403 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: M
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of M CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154419 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF3B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of ORF3B CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154417 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP14
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP14 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154412 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP15
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP15 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154413 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP16
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP16 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154414 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP12
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway-compatible Entry vector, with insert of NSP12 CDS with a TEV sequence at the N-term.; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_154410 Copy
Vector Backbone Description: Backbone Size:7029; Vector Backbone:N/A; Vector Types:S. aureus-E. coli shuttle vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17512993
Comments: pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB.
Proper citation: RRID:Addgene_15457 Copy
Species: Synthetic
Genetic Insert: CPMV (CowPea Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32856047
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.14.095406v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_154597 Copy
Species: E. coli
Genetic Insert: gRNA targeting pgaC
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154919 Copy
Species: E. coli
Genetic Insert: gRNA targeting ampG
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154923 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: N
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_152987 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF10wu
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_152986 Copy
Genetic Insert: CRISPR array
Vector Backbone Description: Vector Backbone:pTF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33710766
Proper citation: RRID:Addgene_153037 Copy
Vector Backbone Description: Backbone Marker:Marillonnet, IPB; Backbone Size:6683; Vector Backbone:pAGM42068; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898975
Comments: Contains a carotenoid biosynthesis cassette that is eliminated during cloning of an insert Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153219 Copy
Species: Arabidopsis thaliana
Genetic Insert: Arabidopsis U6 snRNA promoter
Vector Backbone Description: Backbone Marker:Marillonnet, IPB; Backbone Size:2069; Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898975
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153220 Copy
Species: Synthetic
Genetic Insert: guide RNA constant part, carotenoid marker
Vector Backbone Description: Backbone Marker:Marillonnet, IPB; Backbone Size:2069; Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898975
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153225 Copy
Vector Backbone Description: Backbone Size:4380; Vector Backbone:pMAP; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_153359 Copy
Vector Backbone Description: Backbone Size:4384; Vector Backbone:pMAP; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_153358 Copy
Vector Backbone Description: Backbone Size:4384; Vector Backbone:pMAP; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_153357 Copy
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