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Species: Saccharomyces cerevisiae
Genetic Insert: CHC1
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18850 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Backbone Marker:pRS416; Backbone Size:7497; Vector Backbone:pAG416GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162536
Proper citation: RRID:Addgene_18851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase
Vector Backbone Description: Backbone Marker:Guzman et al.; Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_19041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19111 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19102 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Protein DOM34
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17889667
Proper citation: RRID:Addgene_19109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP70 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBluescript SK+; Vector Types:carries FLP recombinase cDNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS4
Vector Backbone Description: Backbone Size:5037; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21495 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNF7
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS20
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polyA polymerase
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:Pet21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15909992
Comments: See attached purification scheme
Proper citation: RRID:Addgene_21716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ura3
Vector Backbone Description: Backbone Marker:R. Kostriken; Backbone Size:2700; Vector Backbone:pRK113; Vector Types:integration sequence; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3065627
Comments: Constructed by inserting a HindIII URA3 fragment into the HindIII
site of the multiple cloning site of plasmid pRK113.
Contains the 117-bp HO cut site of
MATa inserted between the HincII and BamHI sites of pUC9 and the URA3 1.17-kb HindIII fragment at the HindlIl
site transcribed away from the HO cut site.
Proper citation: RRID:Addgene_21677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fus3-as2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Comments: There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.
Proper citation: RRID:Addgene_21789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dig1-YFP
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Comments: Construction was complicated; please refer to parent article's supplemental materials for details of construction.
Proper citation: RRID:Addgene_21788 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: REV1
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19528320
Comments: The REV1 ORF plus 1050 nucleotides (nt) of its upstream region that include the REV1 promoter and 500nt of the downstream region that include the REV1 transcription terminator was cloned in the vector YCplac111, which carries the LEU2 gene, the autonomous replicating sequence ARS1, and the centromeric CEN4 region.
A BglII restriction site was added by site-directed mutagenesis right before the stop codon of REV1. A 33-mer oligonucleotide duplex containing the HA-coding sequence and a BamHI and a BglII restriction site was inserted into the BglII restriction site, adding an HA tag in fusion with REV1, and regenerating a unique BglII restriction site. This step was repeated three times in order to get four HA tags in fusion with REV1.
Proper citation: RRID:Addgene_22287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Comments: Mutation abolishes the ubiquitin ligase function
Proper citation: RRID:Addgene_22288 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Proper citation: RRID:Addgene_22289 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16980971
Proper citation: RRID:Addgene_25464 Copy
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