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Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203674 Copy
Vector Backbone Description: Backbone Marker:Lidstrom Lab; Backbone Size:7245; Vector Backbone:pCM80; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Comments: The original backbone plasmid, pCM80, is published in Marx & Lidstrom, 2001, Microbiology: Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria.
Proper citation: RRID:Addgene_59395 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59397 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59400 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59774 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59773 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26237426
Proper citation: RRID:Addgene_85496 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12537491
Comments: Please note that this plasmid has been slightly modified from the original version (Mehl et al., 2003).
Proper citation: RRID:Addgene_85502 Copy
Vector Backbone Description: Backbone Marker:Keen et al. (1988) Gene 70:191-197; Backbone Size:22000; Vector Backbone:pLAFR5; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22685282
Comments: For expression in S. meliloti, this is the information on its construction and for cloning ORFs into it.
1) This is the sequence of the SMb21651 promoter fragment (reverse comp of 1683333-1682932) promoter fragment from pSymB of S. meliloti 1021. It spans the sequence at the beginning of the reference sequence of pSymB through the end of the pSymB plasmid:
Primers used to amplify the SMb21651 promoter:
SMb21651 promoter forward primer: 5’ GCaagcttGGCACGCGTCTGAATGTCCATTGT
SMb21651 promoter reverse primer: 5’ GCctgcagGTCGATGTCCGTGCCGCAACT
2) This fragment was amplified and cloned into HindIII/PstI sites of pLAFR5 (Keen et al. (1988) Gene 70:191-197).
The full mcs of pLAFR5 is HindIII—PstI—SalI—BamHI—SmaI (not unique)—EcoRI.
3) ORFs to be expressed in pstb-LAFR5 from the Smb21651 promoter can be cloned into PstI/BamHI
4) Important for cloning ORFs into pstb-LAFR5---Since there are uncharacterized ORFs in this promoter sequence, the forward ORF primer should have stop codons, followed by an rbs:
PstI ____stop codons__ ___rbs and spacer__
ctgcagTAACGTAACGTAAATGGAGTCACCTCT→followed by the ATG of the ORF
(The first use of this promoter [in a different construct] and this 3x stop codon/rbs/spacer design is from Zhang and Cheng. (2006) Applied and Environmental Microbiology 74: 2738-2748
The reverse ORF primer doesn't need anything except a BamHI site.
Proper citation: RRID:Addgene_86169 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL1329 (salA) containing all of RL1328 (salR)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83785 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83784 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL0996 containing all of RL0997
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83782 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: promoter of RL1860
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83778 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: upstream region of RL2720 (rbsC)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83779 Copy
Species: Rhizobium leguminosarum bv. viciae, strain 3841
Genetic Insert: Region upstream RL0990 (matA) containing all of RL0989 (matR)
Vector Backbone Description: Backbone Size:16260; Vector Backbone:pIJ11268; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28495892
Proper citation: RRID:Addgene_83790 Copy
Species: Bacteria
Genetic Insert: Tn7ABCDE
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:2834269
Comments: pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site.
Proper citation: RRID:Addgene_8484 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25853840
Comments: Precursor strain: ElectroMAX DH10B E. coli cells
Landing pad cassette at at the aptI/gidB locus includes in this order: I-SceI restriction site - Landing Pad 1 (LP1) sequence - tetA gene - Landing Pad 2 (LP2) sequence - I-SceI restriction site.
This strain was made based on the technology described in the Kulman and Cox 2010 Nucleic Acids Res. paper: Site-specific chromosomal integration of large synthetic constructs. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847246/
Proper citation: RRID:Addgene_83036 Copy
Vector Backbone Description: Backbone Size:9977; Vector Backbone:pLLX13; Vector Types:Yeast and bacteria shuttle vector, contains origin of transfer for conjugation, can be used as backbone for a capture vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12815109
Comments: Additional reference: Reeves AZ, Spears WE, Du J, Tan KY, Wagers AJ, Lesser CF. (2015) Engineering Escherichia coli into a protein delivery system for mammalian cells. ACS Synthetic Biology, 2015, May 15;4(5):644-54
Proper citation: RRID:Addgene_79825 Copy
Genetic Insert: omega knockout Bacteria hybrid selection strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:XL-1 blue F' episome; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18332042
Comments: This strain has a deletion in hisB, pyrF and ropZ genes (the bacterial homologs of HIS3 and URA3 and the omega subunit) and it contains a F' episome bearing the lacl^q repressor.
Proper citation: RRID:Addgene_18049 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH10
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40861 Copy
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