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Species: Synthetic
Genetic Insert: Twin Streptavidin uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174591 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN7
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Comments: SEPT9 isoform 3 is the protein product of transcript variant 3 (NCBI Reference Sequence: NP_006631.2).
Proper citation: RRID:Addgene_174501 Copy
Species: Synthetic
Genetic Insert: 6xHIS uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174589 Copy
Species: Synthetic
Genetic Insert: Twin Streptavidin uncleavable
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174586 Copy
Species: Synthetic
Genetic Insert: 6xHIS-GB1
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_174587 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174495 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27473911
Comments: For protein expression, grow at 17degC.
Plasmid used in the following publication:
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
Proper citation: RRID:Addgene_174496 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN6
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174499 Copy
Vector Backbone Description: Backbone Size:4506; Vector Backbone:pKO403; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35023783
Proper citation: RRID:Addgene_174726 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn without STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47996/; Vector Backbone:pICSL01005; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174766 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-L17E without STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47996/; Vector Backbone:pICSL01005; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174768 Copy
Species: N. benthamiana
Genetic Insert: GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47998/; Vector Backbone:pICH41308; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174762 Copy
Species: N. benthamiana
Genetic Insert: GG_NbZAR1syn-D481V with STOP codon
Vector Backbone Description: Backbone Marker:https://www.addgene.org/47998/; Vector Backbone:pICH41308; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34633454
Proper citation: RRID:Addgene_174763 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: eGFP-NLS fluorescent reporter
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171316 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: hisB AN6536.2 histidine, selection marker
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171314 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: hisB Pc20g11690 histidine, selection marker
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171313 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: PfraA An16g04690 promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171279 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: sdh-H85L An14g04400 succinate dehydrogenase, selection marker
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171312 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: Patp9 An04g08190 promoter
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171275 Copy
Species: Penicillium, Aspergillus, or other fungal sources
Genetic Insert: eYFP-NLS fluorescent reporter (for fusion)
Vector Backbone Description: Vector Backbone:pAGM1301; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171342 Copy
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