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Species: Arabidopsis thaliana
Genetic Insert: tUBQ1
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3512; Vector Backbone:pCR-BluntII-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37012288
Proper citation: RRID:Addgene_195898 Copy
Species: Arabidopsis thaliana
Genetic Insert: ABI-tagBFP-Sp dCas9
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27776111
Proper citation: RRID:Addgene_84239 Copy
Species: Arabidopsis thaliana
Genetic Insert: ABI-tagBFP-Sa dCas9
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27776111
Proper citation: RRID:Addgene_84243 Copy
Species: Arabidopsis thaliana
Genetic Insert: GAI-tagBFP-Sa dCas9
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27776111
Proper citation: RRID:Addgene_84244 Copy
Species: Arabidopsis thaliana
Genetic Insert: sAPX
Vector Backbone Description: Backbone Marker:pGWB2; Backbone Size:16636; Vector Backbone:pGemini (Dr. Andrew Simkin); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84735 Copy
Species: Arabidopsis thaliana
Genetic Insert: APX1
Vector Backbone Description: Backbone Marker:pGWB2; Backbone Size:16636; Vector Backbone:pGemini (Dr. Andrew Simkin); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84736 Copy
Species: Arabidopsis thaliana
Genetic Insert: sAPX
Vector Backbone Description: Backbone Marker:pGWB2; Backbone Size:16636; Vector Backbone:pGemini (Dr. Andrew Simkin); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84738 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtPCO2
Vector Backbone Description: Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29848548
Proper citation: RRID:Addgene_191799 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtPCO3
Vector Backbone Description: Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29848548
Proper citation: RRID:Addgene_191800 Copy
Species: Arabidopsis thaliana
Genetic Insert: p35S-5UTMV-CTPSSU-mCHERRY-3XFLAGnYFP-3U+TER35S
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pICH47742; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172808 Copy
Species: Arabidopsis thaliana
Genetic Insert: [35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-deltamPTAC5-cYFPX3HA][35S-SP_SV40-CFP]
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM4673; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172819 Copy
Species: Arabidopsis thaliana
Genetic Insert: p35S-5UTMV-OEP7-mTRQ-3U+TER35S
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pICH47761; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172815 Copy
Species: Arabidopsis thaliana
Genetic Insert: p35S-5UTMV-CTPSSU-mCHERRY-cYFP3XHA-3U+TER35S
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pICH47751; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172809 Copy
Species: Arabidopsis thaliana
Genetic Insert: [35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM4673; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172824 Copy
Species: Arabidopsis thaliana
Genetic Insert: [35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM4673; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172820 Copy
Species: Arabidopsis thaliana
Genetic Insert: CmYLCV:Luc+:HSP
Vector Backbone Description: Vector Backbone:pMOD_A4800EC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176878 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10:Luc+:RbcsE9
Vector Backbone Description: Vector Backbone:pMOD_C'4800EC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176879 Copy
Species: Arabidopsis thaliana
Genetic Insert: HSP terminator
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176882 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtAFB2
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6390; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179889 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U3 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35022409
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173156 Copy
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