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Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52716 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN4
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 493–511 from the start sequence of rat α-actinin-4 (the sense chain was 5’-gactatccaggagatgcagttcaagagactgcatctcctggatagtctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52679 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN1
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 476–494 from the start sequence of rat α-actinin-1 (the sense chain was 5’-gatctccaacgtcaacaagttcaagagacttgttgacgttggagatctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52676 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN2
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 224–242 from the start sequence of rat α-actinin-2 (the sense chain was 5’-tggcctgatggatcatgagttcaagagactcatgatccatcaggccatttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52677 Copy
Genetic Insert: G794D strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Proper citation: RRID:Addgene_52710 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52717 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: tetO7-Pcyc1+LINKER
Vector Backbone Description: Vector Backbone:pFA6a-kanMX-tetO-Pcyc1 (41023); Vector Types:Yeast Expression, Promoter replacement; Bacterial Resistance:Ampicillin
Comments: This is the same as the "regular" tetO plasmids (41022, 41023, 41024, 41025, or 41026) with an additional LADYGAG linker. This allows amplification of tagging cassettes from the LINKER vectors using the same pair of primers. For the “regular” vectors the reverse primer is unique to each plasmid.
Proper citation: RRID:Addgene_52685 Copy
Species: Mus musculus
Genetic Insert: ABL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23081945
Comments: Encodes isoform IV of mouse c-Abl, which is N-myristylated.
Proper citation: RRID:Addgene_52684 Copy
Species: Homo sapiens
Genetic Insert: HMGA2
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52727 Copy
Species: Homo sapiens
Genetic Insert: EGR1
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52724 Copy
Species: Homo sapiens
Genetic Insert: HPV26 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24816794
Proper citation: RRID:Addgene_52601 Copy
Species: Homo sapiens
Genetic Insert: HPV66 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24816794
Proper citation: RRID:Addgene_52602 Copy
Species: Homo sapiens
Genetic Insert: HPV31 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24816794
Proper citation: RRID:Addgene_52600 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52721 Copy
Species: Homo sapiens
Genetic Insert: NRAS
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52728 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52729 Copy
Genetic Insert: KIlac strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: KIlac refers to the knock-in of the wild-type operon into the attTn7 locus; this strain was used as the reference strain for all analyses. We constructed all knock-in strains using the approach of McKenzie and Craig. The knock-in contained the entire lac operon starting 75bp upstream of lacI and ending 100bp downstream of lacA. The ΔZYA strain was transformed with pGRG37. The plasmid carried both the lac operon and the transposon genes tnsABCD, which allow for a site-specific insertion at attTn7.
Proper citation: RRID:Addgene_52696 Copy
Genetic Insert: OpLac1 strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: The sequence Oplac1 was synthesized using preferred codons at every position (GENEART), thus optimizing the codon adaptation index.
Proper citation: RRID:Addgene_52697 Copy
Species: Homo sapiens
Genetic Insert: HRAS
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52730 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2736; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii; Bacterial Resistance:Ampicillin
Comments: Cong L, Ran FA, Cox D, Lin S, Barretto R, et al. (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819–823. doi:10.1126/science.1231143.
Proper citation: RRID:Addgene_52694 Copy
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