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Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1, 40a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87894 Copy
Species: Homo sapiens
Genetic Insert: DYSF, alt exon 1, 5a, 17, 40a
Vector Backbone Description: Vector Backbone:pUC57 Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.
Proper citation: RRID:Addgene_87895 Copy
Species: Homo sapiens
Genetic Insert: CD3z (Cytoplasmic domain)
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27056844
Proper citation: RRID:Addgene_78508 Copy
Species: E. coli
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27060147
Proper citation: RRID:Addgene_78492 Copy
Species: Homo sapiens
Genetic Insert: USP19 C506S
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCitrine-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27295555
Proper citation: RRID:Addgene_78594 Copy
Species: Synthetic
Genetic Insert: J101-rbs32-arsR-t-ParsR
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78635 Copy
Species: Synthetic
Genetic Insert: 30hrpR-32hrpS-t-hrpL-30gfp-t
Vector Backbone Description: Backbone Marker:Wang Lab; Vector Backbone:pBW100ParsR; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78637 Copy
Species: Synthetic
Genetic Insert: araC-PBAD
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78639 Copy
Species: Synthetic
Genetic Insert: J106-30hrpR-32hrpS-t-hrpL-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78651 Copy
Species: Synthetic
Genetic Insert: J105-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78644 Copy
Species: Synthetic
Genetic Insert: J106-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78645 Copy
Species: Synthetic
Genetic Insert: J116-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78642 Copy
Species: Synthetic
Genetic Insert: J114-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78641 Copy
Species: Synthetic
Genetic Insert: J114-30hrpR-32hrpS-t-hrpL-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Vector Backbone:pSB3K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030903
Proper citation: RRID:Addgene_78647 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5025; Vector Backbone:pJR255; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_78550 Copy
Species: E. coli
Genetic Insert: Mechanosensitive Channel of Small Conductance
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5364; Vector Backbone:pET28b; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446901
Proper citation: RRID:Addgene_78555 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5025; Vector Backbone:peGFP-N3; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_78549 Copy
Species: Danio rerio
Genetic Insert: kdrl promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2600; Vector Backbone:pDONRp4p1r; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17306248
Proper citation: RRID:Addgene_78687 Copy
Species: Danio rerio
Genetic Insert: acta2 promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2600; Vector Backbone:pDONRp4p1r; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24594685
Comments: The entire intron 1 was inserted upstream of 300 bp of proximal acta2 promoter to make a compact promoter/enhancer construct that could easily be used for transgenesis.
Proper citation: RRID:Addgene_78686 Copy
Genetic Insert: Integration Site 7
Vector Backbone Description: Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27470089
Proper citation: RRID:Addgene_78950 Copy
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