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Vector Backbone Description: Backbone Marker:John Mekalanos lab, Harvard Medical School (PMID: 2836362); Backbone Size:5720; Vector Backbone:pGP704; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers.
Note: Addgene's NGS results found that a large portion of the plasmid backbone (containing the oriT feature) is in the opposite orientation compared to the depositor's reference sequence.
Proper citation: RRID:Addgene_43829 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43891 Copy
Genetic Insert: TALE-activator p65 backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894
Proper citation: RRID:Addgene_43889 Copy
Species: Homo sapiens
Genetic Insert: Human Optimized S. pyogenes Cas9
Vector Backbone Description: Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23460208
Proper citation: RRID:Addgene_43804 Copy
Genetic Insert: SID4X Domain
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22828628
Comments: This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.
Proper citation: RRID:Addgene_43882 Copy
Genetic Insert: TALE-activator VP64 Backbone
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396285
Comments: Location of plasmid features:
EF1a = 922-2106
start codon - 2119-2121
NLS = 2122-2165
N-term =2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
VP64 = 2956-3122
pA = 3125-3244
Proper citation: RRID:Addgene_43885 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8071; Vector Backbone:pLVX-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31346185
Proper citation: RRID:Addgene_164589 Copy
Species: Drosophila melanogaster
Genetic Insert: U6 and DR
Vector Backbone Description: Backbone Size:7350; Vector Backbone:white+attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616113
Proper citation: RRID:Addgene_164586 Copy
Species: Mus musculus
Genetic Insert: Ubiquitin noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164530 Copy
Species: Mus musculus
Genetic Insert: Smoothened noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164533 Copy
Species: Mus musculus
Genetic Insert: Smoothened noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164534 Copy
Species: Mus musculus
Genetic Insert: Smoothened
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164531 Copy
Species: Mus musculus
Genetic Insert: Smoothened
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164532 Copy
Species: Homo sapiens
Genetic Insert: p21 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5600; Vector Backbone:pJFCAT1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796420
Comments: Human p21 promoter deletion-CAT reporter.
Proper citation: RRID:Addgene_16463 Copy
Species: Mus musculus
Genetic Insert: Smoothened W539L
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Comments: SmoM2 protein causes basal cell carcinoma
Proper citation: RRID:Addgene_164537 Copy
Species: Mus musculus
Genetic Insert: Smoothened noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164535 Copy
Species: Homo sapiens
Genetic Insert: Sonic Hedgehog
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164526 Copy
Species: Mus musculus
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164527 Copy
Species: Mus musculus
Genetic Insert: Ubiquitin noK
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164528 Copy
Species: Mus musculus
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32435793
Proper citation: RRID:Addgene_164529 Copy
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