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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pRE107 Resource Report Resource Website 1+ mentions |
RRID:Addgene_43829 | Ampicillin | PMID:9511756 | The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination. Plasmid pRE107 was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis. The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers. Note: Addgene's NGS results found that a large portion of the plasmid backbone (containing the oriT feature) is in the opposite orientation compared to the depositor's reference sequence. | Backbone Marker:John Mekalanos lab, Harvard Medical School (PMID: 2836362); Backbone Size:5720; Vector Backbone:pGP704; Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:10 | 1 | |||
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MLM2579 Resource Report Resource Website |
RRID:Addgene_43891 | TALE-activator p65 backbone | Ampicillin | PMID:23396285 | Location of plasmid features: EF1a = 922-2106 start codon = 2119-2121 NLS = 2122-2165 N-term = 2166-2577 0.5 domain = 2592-2658 C-term = 2665-2949 p65 = 2959-3768 pA = 3775-3894 | Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:10 | 0 | ||
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MLM2583 Resource Report Resource Website |
RRID:Addgene_43889 | TALE-activator p65 backbone | Ampicillin | PMID:23396285 | Location of plasmid features: EF1a = 922-2106 start codon = 2119-2121 NLS = 2122-2165 N-term = 2166-2577 0.5 domain = 2592-2658 C-term = 2665-2949 p65 = 2959-3768 pA = 3775-3894 | Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:10 | 0 | ||
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p415-GalL-Cas9-CYC1t Resource Report Resource Website 10+ mentions |
RRID:Addgene_43804 | Human Optimized S. pyogenes Cas9 | Homo sapiens | Ampicillin | PMID:23460208 | Backbone Size:6670; Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:10 | 18 | ||
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Enhanced Repressor Domain for TAL Effector (SID4X) Resource Report Resource Website 1+ mentions |
RRID:Addgene_43882 | SID4X Domain | Ampicillin | PMID:22828628 | This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article. | Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:10 | 1 | ||
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MLM3585 Resource Report Resource Website |
RRID:Addgene_43885 | TALE-activator VP64 Backbone | Ampicillin | PMID:23396285 | Location of plasmid features: EF1a = 922-2106 start codon - 2119-2121 NLS = 2122-2165 N-term =2166-2577 0.5 domain = 2592-2658 C-term = 2665-2949 VP64 = 2956-3122 pA = 3125-3244 | Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALE Transcriptional Activator Expression Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:10 | 0 | ||
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pLVX-puro_GCaMP6s Resource Report Resource Website 1+ mentions |
RRID:Addgene_164589 | GCaMP6s | Synthetic | Ampicillin | PMID:31346185 | Backbone Marker:Clontech; Backbone Size:8071; Vector Backbone:pLVX-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:22 | 4 | ||
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OA-1043 Resource Report Resource Website 1+ mentions |
RRID:Addgene_164586 | U6 and DR | Drosophila melanogaster | Ampicillin | PMID:33616113 | Backbone Size:7350; Vector Backbone:white+attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:22 | 1 | ||
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PD13 Resource Report Resource Website |
RRID:Addgene_164530 | Ubiquitin noK | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | All seven Lysines of Ubiquitin changed to Arginine | 2026-08-15 01:09:21 | 0 | |
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GP777 Resource Report Resource Website |
RRID:Addgene_164533 | Smoothened noK | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | All 21 cytoplasmic Lysines of Smoothened changed to Arginine | 2026-08-15 01:09:21 | 0 | |
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GP806 Resource Report Resource Website |
RRID:Addgene_164534 | Smoothened noK | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | All 21 cytoplasmic Lysines of Smoothened changed to Arginine | 2026-08-15 01:09:21 | 0 | |
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PD22 Resource Report Resource Website |
RRID:Addgene_164531 | Smoothened | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:09:21 | 0 | |
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GP736 Resource Report Resource Website |
RRID:Addgene_164532 | Smoothened | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:09:21 | 0 | |
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p21 promoter B Resource Report Resource Website |
RRID:Addgene_16463 | p21 promoter | Homo sapiens | Ampicillin | PMID:7796420 | Human p21 promoter deletion-CAT reporter. | Backbone Marker:ATCC; Backbone Size:5600; Vector Backbone:pJFCAT1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:22 | 0 | |
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GP735 Resource Report Resource Website |
RRID:Addgene_164537 | Smoothened W539L | Mus musculus | Ampicillin | PMID:32435793 | SmoM2 protein causes basal cell carcinoma | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | W539L | 2026-08-15 01:09:21 | 0 |
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GP807 Resource Report Resource Website |
RRID:Addgene_164535 | Smoothened noK | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | All 21 cytoplasmic Lysines of Smoothened and all seven lysines of Ubiquitin changed to Arginine | 2026-08-15 01:09:21 | 0 | |
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MS03 Resource Report Resource Website |
RRID:Addgene_164526 | Sonic Hedgehog | Homo sapiens | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:09:21 | 0 | |
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GP734 Resource Report Resource Website |
RRID:Addgene_164527 | Ubiquitin | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:09:21 | 0 | |
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PD19 Resource Report Resource Website |
RRID:Addgene_164528 | Ubiquitin noK | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | All seven Lysines of Ubiquitin changed to Arginine | 2026-08-15 01:09:21 | 0 | |
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PD14 Resource Report Resource Website |
RRID:Addgene_164529 | Ubiquitin | Mus musculus | Ampicillin | PMID:32435793 | Vector Backbone:Phage lentivirus; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | WT | 2026-08-15 01:09:21 | 0 |
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