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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro Resource Report Resource Website 10+ mentions |
RRID:Addgene_141371 | SARS-CoV-2-nsp5-C145A | SARS-CoV-2 | Ampicillin | PMID:32353859 | Please visit https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3 for bioRxiv preprint. | Backbone Marker:Takara Bio; Backbone Size:8825; Vector Backbone:pLVX-EF1alpha-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | C145A, human codon optimized | 2026-08-15 01:06:48 | 10 |
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pDONR223 SARS-CoV-2 NSP14 Resource Report Resource Website 1+ mentions |
RRID:Addgene_141267 | NSP14 | SARS-CoV-2 isolate Wuhan-Hu-1 | Spectinomycin | PMID:32763951 | Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Many synonymous changes due to codon optimization | 2026-08-15 01:06:47 | 3 | |
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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro Resource Report Resource Website 10+ mentions |
RRID:Addgene_141383 | SARS-CoV-2-orf3a | SARS-CoV-2 | Ampicillin | PMID:32353859 | Please visit https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3 for bioRxiv preprint. | Backbone Marker:Takara Bio; Backbone Size:8825; Vector Backbone:pLVX-EF1alpha-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | human codon optimized | 2026-08-15 01:06:48 | 13 |
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pDONR223 SARS-CoV-2 ORF7B Resource Report Resource Website 1+ mentions |
RRID:Addgene_141277 | ORF7B | SARS-CoV-2 isolate Wuhan-Hu-1 | Spectinomycin | PMID:32763951 | Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Many synonymous changes due to codon optimization | 2026-08-15 01:06:47 | 2 | |
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pDONR207 SARS-CoV-2 E Resource Report Resource Website 1+ mentions |
RRID:Addgene_141273 | E | SARS-CoV-2 isolate Wuhan-Hu-1 | Gentamicin | PMID:32763951 | Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin | Many synonymous changes due to codon optimization | 2026-08-15 01:06:47 | 7 | |
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pDONR223 SARS-CoV-2 ORF6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_141275 | ORF6 | SARS-CoV-2 isolate Wuhan-Hu-1 | Spectinomycin | PMID:32763951 | Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Many synonymous changes due to codon optimization | 2026-08-15 01:06:47 | 1 | |
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pDONR207 SARS-CoV-2 ORF7A Resource Report Resource Website 1+ mentions |
RRID:Addgene_141276 | ORF7A | SARS-CoV-2 isolate Wuhan-Hu-1 | Gentamicin | PMID:32763951 | Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin | Many synonymous changes due to codon optimization | 2026-08-15 01:06:47 | 2 | |
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pDONR223 SARS-CoV-2 ORF3B Resource Report Resource Website 1+ mentions |
RRID:Addgene_141272 | ORF3B | SARS-CoV-2 isolate Wuhan-Hu-1 | Spectinomycin | PMID:32763951 | Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Many synonymous changes due to codon optimization | 2026-08-15 01:06:47 | 1 | |
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pGWB401NL3F10H Resource Report Resource Website 1+ mentions |
RRID:Addgene_141285 | Chloramphenicol and Spectinomycin | PMID:31316580 | Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium). | Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin | 2026-08-15 01:06:47 | 2 | |||
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pGWB501NL3F10H Resource Report Resource Website 1+ mentions |
RRID:Addgene_141286 | Chloramphenicol and Spectinomycin | PMID:31316580 | Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium). | Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin | 2026-08-15 01:06:47 | 1 | |||
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pGWB601NL3F10H Resource Report Resource Website 1+ mentions |
RRID:Addgene_141287 | Chloramphenicol and Spectinomycin | PMID:31316580 | Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium). | Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin | 2026-08-15 01:06:47 | 1 | |||
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pLD-puro-Cc-TARDBP-WT_VA Resource Report Resource Website 1+ mentions |
RRID:Addgene_141327 | TARDBP-WT | Homo sapiens | Ampicillin | PMID:32325033 | Backbone Marker:Addgene# 24588; Backbone Size:9389; Vector Backbone:pLD-puro-Cc-VA (MAPLE vector); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:48 | 1 | ||
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pET28a(+)::MBP-NL3F10H Resource Report Resource Website 1+ mentions |
RRID:Addgene_141291 | MBP-NLUC3F10H | Synthetic | Chloramphenicol and Kanamycin | PMID:31316580 | Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin | Engineered for high stability (t1/2 = 11.5 days at 37 °C) and codon optimised for expression in mammalian cells. | 2026-08-15 01:06:47 | 1 | |
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GB1-PRD(703-800)-Strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_141344 | GB1-His6-TEV-ALIX(703-800)-Strep | Homo sapiens | Ampicillin | PMID:32917811 | Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:48 | 1 | ||
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pAG305GAL-ccdB Resource Report Resource Website 1+ mentions |
RRID:Addgene_14137 | Chloramphenicol and Ampicillin | PMID:17583893 | Type of plasmid: integrating. This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid. | Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:06:48 | 3 | |||
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pAG305GPD-ccdB Resource Report Resource Website 1+ mentions |
RRID:Addgene_14138 | Chloramphenicol and Ampicillin | PMID:17583893 | Type of plasmid: integrating. This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid. | Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:06:48 | 1 | |||
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pAG304GPD-ccdB Resource Report Resource Website 1+ mentions |
RRID:Addgene_14136 | Chloramphenicol and Ampicillin | PMID:17583893 | Type of plasmid: integrating. This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid. | Backbone Size:0; Vector Backbone:pRS304; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:06:48 | 2 | |||
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pAG303GAL-ccdB Resource Report Resource Website 1+ mentions |
RRID:Addgene_14133 | Chloramphenicol and Ampicillin | PMID:17583893 | Type of plasmid: integrating. This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid. | Backbone Size:0; Vector Backbone:pRS303; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:06:48 | 3 | |||
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pLX302 EGFP-V5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_141348 | EGFP | Synthetic | Ampicillin | Backbone Marker:Addgene #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:06:48 | 4 | |||
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pAG306GPD-ccdB-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_14188 | Chloramphenicol and Ampicillin | PMID:17583893 | Type of plasmid: integrating. This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid. | Backbone Size:0; Vector Backbone:pRS306; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:06:49 | 1 |
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