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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
BII-BCA-Prom-ASCL2
 
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RRID:Addgene_133394 destabilized tdTomato-NLS and destabilized NLS-GFP Homo sapiens Ampicillin PMID:30017821 Backbone Marker:Madison Lab; Vector Backbone:BII-BCA-Prom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N/A 2026-08-15 01:05:35 0
pGEX-2TKN-bp1
 
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RRID:Addgene_133428 SREBF1 Homo sapiens Ampicillin PMID:16799563 HMS clone ID number: HsCD00063793. Vector Backbone:pGEX-2TKN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin SREBP1a amino acids 1-50 fused to GST 2026-08-15 01:05:35 0
pHIS-GB1-BP1
 
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RRID:Addgene_133427 SREBF1 Homo sapiens Ampicillin HMS clone ID number: HsCD00063792. Vector Backbone:pHIS-GB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin SREBP1a amino acids 17-42 fused to GB1 solubility tag 2026-08-15 01:05:35 0
BII-ChiNW-ASCL2
 
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1+ mentions
RRID:Addgene_133384 ASCL2 Homo sapiens Ampicillin PMID:37310750 Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint. Backbone Marker:Madison Lab; Vector Backbone:BII-ChiNW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N/A 2026-08-15 01:05:35 1
BII-ChBtW-ASCL2
 
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RRID:Addgene_133375 ASCL2 Homo sapiens Ampicillin PMID:37310750 Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint. Backbone Marker:Madison Lab; Vector Backbone:BII-ChBtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N/A 2026-08-15 01:05:35 0
BII-ChBtW-IGF2
 
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RRID:Addgene_133374 IGF2 Homo sapiens Ampicillin PMID:37310750 Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint. Backbone Marker:Madison Lab; Vector Backbone:BII-ChBtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N/A 2026-08-15 01:05:35 0
human ETV6 gRNA-3
 
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RRID:Addgene_133403 ETV6 Homo sapiens Kanamycin PMID:29554589 Backbone Marker:George Church; Backbone Size:3915; Vector Backbone:gRNA_Cloning Vector(Addgene#41824); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:05:35 0
BII-ChBtW-PLAGL2
 
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RRID:Addgene_133369 PLAGL2 Homo sapiens Ampicillin PMID:37310750 Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint. Backbone Marker:Madison Lab; Vector Backbone:BII-ChBtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N/A 2026-08-15 01:05:35 0
ps226
 
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RRID:Addgene_133557 CDKN1A Homo sapiens Ampicillin PMID:15655109 HMS clone ID number: HsSH00021241. Ho Man Chan made the clone. According to the clone donor, NOT identical to the shRNA reported in the paper (that is ps225) but this is a functional shRNA against ps226. Backbone Size:6349; Vector Backbone:pRETRO-SUPER; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:05:37 0
pLB(N)CX AT-CH3-FLAG-HA WY
 
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RRID:Addgene_133725 EP300 Homo sapiens Ampicillin PMID:16611888 HMS clone ID number: HsCD00061647. Please see Supplemental Documents > Depositors Notes for more cloning information. Backbone Size:6229; Vector Backbone:pLB(N)CX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin partial (not complete) p300 fragment, AT and CH3 domains only; W1466A, Y1467S 2026-08-15 01:05:38 0
pET30 SUMO-1 G97
 
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RRID:Addgene_13381 SUMO-1, G97 Homo sapiens Kanamycin PMID:9427648 Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin G97 mutant: lacks 4 amino acids from the C-terminus 2026-08-15 01:05:39 0
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
 
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RRID:Addgene_133779 Isocitrate dehydrogenase 1 Homo sapiens Kanamycin Backbone Size:4828; Vector Backbone:pKT; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Arginine is mutated to histidine at amino acid position 132 on IDH1 2026-08-15 01:05:39 0
pcDNA3-6Xhis-human SUMO 3
 
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RRID:Addgene_133772 SUMO 3 Homo sapiens Ampicillin PMID:15897463 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA 3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:05:39 0
pcDNA3-6XHis-human SUMO 2
 
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1+ mentions
RRID:Addgene_133771 SUMO 2 Homo sapiens Ampicillin PMID:15897463 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA 3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:05:39 2
pLVX-Puro-TDP-43-P112H
 
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RRID:Addgene_133760 TDP-43 Homo sapiens Ampicillin PMID:31527135 Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint. Backbone Marker:clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Changed proline 112 to histidine. 2026-08-15 01:05:39 0
pLVX-Puro-TDP-43-M337V
 
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RRID:Addgene_133759 TDP-43 Homo sapiens Ampicillin PMID:31527135 Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint. Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Changed methionine 337 to valine 2026-08-15 01:05:39 0
pLVX-Puro-TDP-43-K263E
 
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RRID:Addgene_133758 TDP-43 Homo sapiens Ampicillin PMID:31527135 Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint. Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Changed lysine 263 to glutamate. 2026-08-15 01:05:39 0
PWPXLD-EGFR-WT
 
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1+ mentions
RRID:Addgene_133749 Wild type EGFR-HA Homo sapiens Ampicillin PMID:31433979 Backbone Size:10455; Vector Backbone:PWPXLD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:05:39 2
MSCV-human CSFI-R-L301S-IRES-GFP
 
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RRID:Addgene_133744 CSF1-R Homo sapiens Ampicillin PMID:3027579 Backbone Size:6415; Vector Backbone:MSCV LTR ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin L301S 2026-08-15 01:05:39 0
AICSDP-82:SON-mEGFP
 
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1+ mentions
RRID:Addgene_133964 SON Homology Arms with mEGFP-linker Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human SON in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) 2026-08-15 01:05:41 1

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