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Species: Homo sapiens
Genetic Insert: destabilized tdTomato-NLS and destabilized NLS-GFP
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:BII-BCA-Prom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30017821
Proper citation: RRID:Addgene_133394 Copy
Species: Homo sapiens
Genetic Insert: SREBF1
Vector Backbone Description: Vector Backbone:pGEX-2TKN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16799563
Comments: HMS clone ID number: HsCD00063793.
Proper citation: RRID:Addgene_133428 Copy
Species: Homo sapiens
Genetic Insert: SREBF1
Vector Backbone Description: Vector Backbone:pHIS-GB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: HMS clone ID number: HsCD00063792.
Proper citation: RRID:Addgene_133427 Copy
Species: Homo sapiens
Genetic Insert: ASCL2
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:BII-ChiNW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37310750
Comments: Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint.
Proper citation: RRID:Addgene_133384 Copy
Species: Homo sapiens
Genetic Insert: ASCL2
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:BII-ChBtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37310750
Comments: Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint.
Proper citation: RRID:Addgene_133375 Copy
Species: Homo sapiens
Genetic Insert: IGF2
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:BII-ChBtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37310750
Comments: Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint.
Proper citation: RRID:Addgene_133374 Copy
Species: Homo sapiens
Genetic Insert: ETV6
Vector Backbone Description: Backbone Marker:George Church; Backbone Size:3915; Vector Backbone:gRNA_Cloning Vector(Addgene#41824); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29554589
Proper citation: RRID:Addgene_133403 Copy
Species: Homo sapiens
Genetic Insert: PLAGL2
Vector Backbone Description: Backbone Marker:Madison Lab; Vector Backbone:BII-ChBtW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37310750
Comments: Please visit https://doi.org/10.1101/2020.10.14.339531 for bioRxiv preprint.
Proper citation: RRID:Addgene_133369 Copy
Species: Homo sapiens
Genetic Insert: CDKN1A
Vector Backbone Description: Backbone Size:6349; Vector Backbone:pRETRO-SUPER; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15655109
Comments: HMS clone ID number: HsSH00021241.
Ho Man Chan made the clone.
According to the clone donor, NOT identical to the shRNA reported in the paper (that is ps225) but this is a functional shRNA against ps226.
Proper citation: RRID:Addgene_133557 Copy
Species: Homo sapiens
Genetic Insert: EP300
Vector Backbone Description: Backbone Size:6229; Vector Backbone:pLB(N)CX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16611888
Comments: HMS clone ID number: HsCD00061647.
Please see Supplemental Documents > Depositors Notes for more cloning information.
Proper citation: RRID:Addgene_133725 Copy
Species: Homo sapiens
Genetic Insert: SUMO-1, G97
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9427648
Proper citation: RRID:Addgene_13381 Copy
Species: Homo sapiens
Genetic Insert: Isocitrate dehydrogenase 1
Vector Backbone Description: Backbone Size:4828; Vector Backbone:pKT; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_133779 Copy
Species: Homo sapiens
Genetic Insert: SUMO 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA 3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15897463
Proper citation: RRID:Addgene_133772 Copy
Species: Homo sapiens
Genetic Insert: SUMO 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA 3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15897463
Proper citation: RRID:Addgene_133771 Copy
Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31527135
Comments: Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_133760 Copy
Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31527135
Comments: Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_133759 Copy
Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31527135
Comments: Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_133758 Copy
Species: Homo sapiens
Genetic Insert: Wild type EGFR-HA
Vector Backbone Description: Backbone Size:10455; Vector Backbone:PWPXLD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31433979
Proper citation: RRID:Addgene_133749 Copy
Species: Homo sapiens
Genetic Insert: CSF1-R
Vector Backbone Description: Backbone Size:6415; Vector Backbone:MSCV LTR ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3027579
Proper citation: RRID:Addgene_133744 Copy
Species: Homo sapiens
Genetic Insert: SON Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human SON in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_133964 Copy
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