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Species: Mus musculus
Genetic Insert: IRS-4
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: Mouse IRS-4 genomic DNA was screened from the BAC genomic DNA library (Genome Systems Inc. St. Louis, Mo.) by PCR with specific primers for mouse IRS-4 genomic DNA. The AvrII-ClaI fragment encompassing the entire exon 1 of mouse IRS-4 gene was blunt ended and ligated into the SnaBI site in pBABE-puro.
Proper citation: RRID:Addgene_11363 Copy
Species: Mus musculus
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Mark Van Delft; Backbone Size:6995; Vector Backbone:pMIH/MSCV-IRES-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29472455
Proper citation: RRID:Addgene_113704 Copy
Species: Mus musculus
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: (Kahn lab plasmid name = pBabe puro IRS-1 Myc)
Proper citation: RRID:Addgene_11374 Copy
Species: Mus musculus
Genetic Insert: miR-181a-1
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11376 Copy
Species: Mus musculus
Genetic Insert: IRS-2
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: (Kahn lab plasmid name = pBabe puro mIRS2)
Proper citation: RRID:Addgene_11371 Copy
Species: Mus musculus
Genetic Insert: IRS-2
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: (Kahn lab plasmid name = pBabe puro IRS2 Myc)
Addgene NGS results found a G384E variant and an S172 insertion compared to the NCBI reference [NP_001074681.1].
Proper citation: RRID:Addgene_11373 Copy
Species: Mus musculus
Genetic Insert: IRS-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: (Kahn lab plasmid name = pBS IRS2)
Proper citation: RRID:Addgene_11372 Copy
Species: Mus musculus
Genetic Insert: miR-142
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11377 Copy
Species: Mus musculus
Genetic Insert: TRAF5
Vector Backbone Description: Backbone Size:5086; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19409903
Proper citation: RRID:Addgene_102679 Copy
Species: Mus musculus
Genetic Insert: EphA3
Vector Backbone Description: Vector Backbone:pAd Easy1; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20960543
Comments: This construct is a adenoviral vector which carries mouse EphA3 cDNA and ready to produce adenovirus which express kinase dead (K657M) EphA3 by transfection into 293A cells followed by PacI digestion
Proper citation: RRID:Addgene_102726 Copy
Species: Mus musculus
Genetic Insert: EphA3
Vector Backbone Description: Vector Backbone:pAd Easy1; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20960543
Comments: This construct is a adenoviral vector which carries mouse EphA3 cDNA and ready to produce adenovirus which express wild type EphA3 by transfection into 293A cells followed by PacI digestion
Proper citation: RRID:Addgene_102725 Copy
Species: Mus musculus
Genetic Insert: EphA2 N terminal (1-427aa)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26744526
Proper citation: RRID:Addgene_102737 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha
Vector Backbone Description: Backbone Marker:Amersham Biosciences; Backbone Size:4900; Vector Backbone:pGEX-5x-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10558993
Proper citation: RRID:Addgene_1028 Copy
Species: Mus musculus
Genetic Insert: TACO/Coronin 1
Vector Backbone Description: Backbone Size:6189; Vector Backbone:pCB6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800061
Comments: The C-terminally hemagglutinin (HA)-tagged version of coronin 1 (Cor1-HA) was generated by PCR, by using as template the coronin 1 cDNA cloned into pBluescript via the EcoRI site (Veithen et al., 1996; Ferrari et al., 1999).
The forward primer was
5'-ACAAGCAGCGGAGTCCTGCTACC-3'
(coronin 1 nucleotides 796–818)
and the EcoRI site-containing reverse primer was
5'-CGCGAATTCCTATGCGTAGTCTGGTACGTCGTATGGGTACTTGGCCTGAACAGTCTC-3'
encoding the HA tag and the EcoRI site.
The product was digested with HindIII and EcoRI, and the HindIII/EcoRI fragment was used for exchanging the corresponding wild-type fragment in the pBluescript construct.
For expression in mammalian cells, the construct was cloned via the EcoRI site into the cytomegalovirus promoter-driven pCB6 expression vector.
Proper citation: RRID:Addgene_102815 Copy
Species: Mus musculus
Genetic Insert: Frizzled 3
Vector Backbone Description: Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:10065; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28885975
Proper citation: RRID:Addgene_102865 Copy
Species: Mus musculus
Genetic Insert: Frizzled 9
Vector Backbone Description: Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:10065; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28885975
Proper citation: RRID:Addgene_102877 Copy
Species: Mus musculus
Genetic Insert: IRF8
Vector Backbone Description: Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29587652
Proper citation: RRID:Addgene_102872 Copy
Species: Mus musculus
Genetic Insert: Mouse Kif26b
Vector Backbone Description: Backbone Marker:Addgene #52961 (provided by Feng Zhang); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28885975
Proper citation: RRID:Addgene_102858 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha
Vector Backbone Description: Backbone Marker:Amersham Biosciences; Backbone Size:4900; Vector Backbone:pGEX-5x-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10558993
Proper citation: RRID:Addgene_1029 Copy
Species: Mus musculus
Genetic Insert: Mouse Kif26b
Vector Backbone Description: Backbone Marker:Addgene #52961 (provided by Feng Zhang); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28885975
Proper citation: RRID:Addgene_102857 Copy
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