Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7649; Vector Backbone:pET28-MKH8SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please see http://www.thesgc.org/sites/default/files/toronto_vectors/pET28-MKH8SUMO.pdf for more information.
The pET28-MKH8SUMO vector was derived from expression plasmid pET28a-LIC (SGC). It is used for T7 promoter driven expression of recombinant proteins with the addition of an N-terminal fusion tag containing 8X His followed by a thrombin cleavage site, a SUMO, and a TEV cleavage site. Two lysines were encoded after the Met start site. Two stop codons are included in the vector at the downstream cloning site.
Insertion of DNA sequence into the cloning/expression region is performed using ClonTech In-Fusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI/BsaI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.The 15-bp primer extensions are compatible with those for pET28-MHL, such that the PCR inserts prepared for pET28-MHL can be directly used for cloning into pET28-MKH8SUMO.
Proper citation: RRID:Addgene_79526 Copy
Species: Homo sapiens
Genetic Insert: PGK Promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4765; Vector Backbone:pDONR221-P1P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human PGK Promoter. First Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79489 Copy
Species: Homo sapiens
Genetic Insert: LXR Alpha
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for NR1H3 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79514 Copy
Species: Homo sapiens
Genetic Insert: EMG1
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please see http://www.thesgc.org/structures/5FAI for more information.
Proper citation: RRID:Addgene_79519 Copy
Species: Homo sapiens
Genetic Insert: JMJD1C
Vector Backbone Description: Backbone Marker:SGC; Addgene Plasmid #26105; Backbone Size:7260; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 or other strain with T7 RNA polymerase for protein expression. For more information, please see: http://www.thesgc.org/structures/5FZO
Proper citation: RRID:Addgene_79644 Copy
Species: Homo sapiens
Genetic Insert: Rab4a
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagBFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27110488
Proper citation: RRID:Addgene_79799 Copy
Species: Homo sapiens
Genetic Insert: Rab11a
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27110488
Proper citation: RRID:Addgene_79806 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27110488
Proper citation: RRID:Addgene_79802 Copy
Species: Homo sapiens
Genetic Insert: Rab11a
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagBFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27110488
Proper citation: RRID:Addgene_79805 Copy
Species: Homo sapiens
Genetic Insert: Rab7L1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27110488
Proper citation: RRID:Addgene_79804 Copy
Genetic Insert: Tre-C04
Vector Backbone Description: Backbone Size:3712; Vector Backbone:pTKEI-Dest; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27470466
Proper citation: RRID:Addgene_79754 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pCB301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17720752
Proper citation: RRID:Addgene_80083 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPX domain of the Vacuolar Transporter Chaperone 4
Vector Backbone Description: Backbone Marker:EMBL pepcore; Backbone Size:5502; Vector Backbone:pET-24d modified; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27774698
Proper citation: RRID:Addgene_82337 Copy
Species: Danio rerio
Genetic Insert: mnx1 promoter
Vector Backbone Description: Vector Backbone:pDONRP4-P1r; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82402 Copy
Genetic Insert: tdTomato (lacks stop codon)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82404 Copy
Vector Backbone Description: Vector Backbone:pDON221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82406 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-73 b isoform
Vector Backbone Description: Vector Backbone:pDONR-221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27274054
Proper citation: RRID:Addgene_82362 Copy
Species: Synthetic
Genetic Insert: mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
Vector Backbone Description: Backbone Size:5296; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28851864
Comments: In the mNG2(1-10) fragment, mutations are K128M, S142T, R150M, G172V and K213M; in the mNG2(11) fragment, mutation is V15M.
Proper citation: RRID:Addgene_82611 Copy
Species: Homo sapiens
Genetic Insert: sfCherry2(11)_Keratin
Vector Backbone Description: Backbone Marker:Michael Davidson Fluorescent Protein Collection at the UCSF Nikon Imaging center; Backbone Size:5000; Vector Backbone:pEGFP-mEmerald-Keratin14-N-18; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_82604 Copy
Genetic Insert: scFvGCN4sfGFPTET1CD
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27571369
Proper citation: RRID:Addgene_82561 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
