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Species: Mus musculus
Genetic Insert: hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33510438
Comments: Please note Addgene NGS identified two mutations in mCherry (K14E, N210D) and one in KASH domain of Nesprin2 E>G. Depositor confirms plasmid is functional.
Proper citation: RRID:Addgene_164967 Copy
Species: Danio rerio
Genetic Insert: kcna1a
Vector Backbone Description: Backbone Size:4179; Vector Backbone:pDestTol2pA2; Vector Types:Tol2 transposon destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32546498
Comments: The DNA sequence was amplified from pooled wild type TAB zebrafish embryos. There is a likely polymorphism, K278M, which is not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11.
Proper citation: RRID:Addgene_164965 Copy
Species: Synthetic
Genetic Insert: SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379961
Proper citation: RRID:Addgene_179685 Copy
Species: Synthetic
Genetic Insert: COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379961
Proper citation: RRID:Addgene_179680 Copy
Species: Synthetic
Genetic Insert: COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379961
Proper citation: RRID:Addgene_179683 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5769; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179561 Copy
Species: M13 phage
Genetic Insert: [SD8] gIII
Vector Backbone Description: Vector Backbone:SC101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379961
Proper citation: RRID:Addgene_179678 Copy
Species: Synthetic
Genetic Insert: Caged cTVMVP
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179672 Copy
Species: Synthetic
Genetic Insert: nTVMVP
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179673 Copy
Species: Homo sapiens
Genetic Insert: NLS-NG-T2A-hSHH
Vector Backbone Description: Vector Backbone:KS(-); Vector Types:piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34799555
Proper citation: RRID:Addgene_179586 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7986; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179581 Copy
Species: Homo sapiens
Genetic Insert: death domain associated protein
Vector Backbone Description: Vector Backbone:pGEX-1ZT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34408321
Proper citation: RRID:Addgene_179619 Copy
Genetic Insert: Chrimson
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6771; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179611 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7556; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179575 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Nucleocapsid (N protein)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4760; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Expresses SARS CoV-2 Nucleoprotein (N gene; nucleocapsid) in insect cells with TEV-cleavable N-terminal His6 tag. Express 4-5 days at 27C. Yield from NiNTA column is approximately 100 mg/L of culture using ExpiSF expression system.
Proper citation: RRID:Addgene_179696 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9184; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179576 Copy
Species: Homo sapiens
Genetic Insert: v-myc myelocytomatosis viral oncogene homolog (avian)
Vector Backbone Description: Backbone Size:4622; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18287077
Proper citation: RRID:Addgene_17966 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5767; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179573 Copy
Species: Escherichia coli (strain K12)
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pMAL-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Express MBP-BirA fusion protein overnight at 18C. Lysis buffer: 50 mM Tris pH 8, 300 mM NaCl. Size exclusion buffer: 50 mM Tris pH 8.0, 150 mM NaCl.
Proper citation: RRID:Addgene_179694 Copy
Species: M13 phage
Genetic Insert: [SD8] gIII
Vector Backbone Description: Vector Backbone:SC101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35379961
Comments: [SD8] DddI-VSV-G
Proper citation: RRID:Addgene_179693 Copy
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