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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET28-MKH8SUMO Resource Report Resource Website 10+ mentions |
RRID:Addgene_79526 | Kanamycin | Please see http://www.thesgc.org/sites/default/files/toronto_vectors/pET28-MKH8SUMO.pdf for more information. The pET28-MKH8SUMO vector was derived from expression plasmid pET28a-LIC (SGC). It is used for T7 promoter driven expression of recombinant proteins with the addition of an N-terminal fusion tag containing 8X His followed by a thrombin cleavage site, a SUMO, and a TEV cleavage site. Two lysines were encoded after the Met start site. Two stop codons are included in the vector at the downstream cloning site. Insertion of DNA sequence into the cloning/expression region is performed using ClonTech In-Fusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI/BsaI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.The 15-bp primer extensions are compatible with those for pET28-MHL, such that the PCR inserts prepared for pET28-MHL can be directly used for cloning into pET28-MKH8SUMO. | Backbone Marker:SGC; Backbone Size:7649; Vector Backbone:pET28-MKH8SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:31 | 11 | ||||
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pENTR221-PGK.Pro Resource Report Resource Website |
RRID:Addgene_79489 | PGK Promoter | Homo sapiens | Kanamycin | PMID:27694838 | Multisite Gateway Entry Clone for human PGK Promoter. First Fragment for 2 Fragment MultiSite Gateway LR reaction. | Backbone Marker:Thermo Scientific; Backbone Size:4765; Vector Backbone:pDONR221-P1P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:31 | 0 | |
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pENTR221-NR1H3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_79514 | LXR Alpha | Homo sapiens | Kanamycin | PMID:27694838 | Multisite Gateway Entry Clone for NR1H3 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction. | Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:31 | 1 | |
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5FAI (EMG1) Resource Report Resource Website |
RRID:Addgene_79519 | EMG1 | Homo sapiens | Kanamycin | Please see http://www.thesgc.org/structures/5FAI for more information. | Backbone Marker:SGC (Addgene Plasmid #26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:31 | 0 | ||
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JMJD1CA-c089 Resource Report Resource Website |
RRID:Addgene_79644 | JMJD1C | Homo sapiens | Kanamycin | Use BL21(DE3)-R3-pRARE2 or other strain with T7 RNA polymerase for protein expression. For more information, please see: http://www.thesgc.org/structures/5FZO | Backbone Marker:SGC; Addgene Plasmid #26105; Backbone Size:7260; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:32 | 0 | ||
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pTag-BFP-C-h-Rab4a-c-Myc Resource Report Resource Website |
RRID:Addgene_79799 | Rab4a | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagBFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:33 | 0 | ||
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pTag-RFP-C-h-Rab11a Resource Report Resource Website 1+ mentions |
RRID:Addgene_79806 | Rab11a | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:33 | 1 | ||
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pTag-RFP-C-h-Rab5a-c-Myc Resource Report Resource Website |
RRID:Addgene_79802 | Rab5a | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:33 | 0 | ||
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pTag-BFP-C-h-Rab11a Resource Report Resource Website |
RRID:Addgene_79805 | Rab11a | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagBFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:33 | 0 | ||
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pTag-RFP-C-h-Rab7L1-c-Myc Resource Report Resource Website |
RRID:Addgene_79804 | Rab7L1 | Homo sapiens | Kanamycin | PMID:27110488 | Backbone Marker:Evrogen; Vector Backbone:pTagRFP-C vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:33 | 0 | ||
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pTKEI-Tre-C04 Resource Report Resource Website 1+ mentions |
RRID:Addgene_79754 | Tre-C04 | Kanamycin | PMID:27470466 | Backbone Size:3712; Vector Backbone:pTKEI-Dest; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:33 | 4 | |||
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pJL-TRBO-G Resource Report Resource Website 1+ mentions |
RRID:Addgene_80083 | GFP | Other | Kanamycin | PMID:17720752 | Vector Backbone:pCB301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:36 | 7 | ||
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pMH-macroHC Resource Report Resource Website |
RRID:Addgene_82337 | SPX domain of the Vacuolar Transporter Chaperone 4 | Saccharomyces cerevisiae | Kanamycin | PMID:27774698 | Backbone Marker:EMBL pepcore; Backbone Size:5502; Vector Backbone:pET-24d modified; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Truncated protein, amino acids 1-178 | 2026-08-15 01:20:55 | 0 | |
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p5E-mnx1 Resource Report Resource Website |
RRID:Addgene_82402 | mnx1 promoter | Danio rerio | Kanamycin | PMID:27500400 | Vector Backbone:pDONRP4-P1r; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:58 | 0 | ||
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pME-tdTomato no stop Resource Report Resource Website 1+ mentions |
RRID:Addgene_82404 | tdTomato (lacks stop codon) | Kanamycin | PMID:27500400 | Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:56 | 1 | |||
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pME-FlEx switch Resource Report Resource Website |
RRID:Addgene_82406 | Kanamycin | PMID:27500400 | Vector Backbone:pDON221; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:58 | 0 | ||||
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TU#1448_unc-73b_cDNA Resource Report Resource Website |
RRID:Addgene_82362 | unc-73 b isoform | Caenorhabditis elegans | Kanamycin | PMID:27274054 | Vector Backbone:pDONR-221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:55 | 0 | ||
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pET_mNG2(1-10)_32aalinker_mNG2(11) Resource Report Resource Website 1+ mentions |
RRID:Addgene_82611 | mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11) | Synthetic | Kanamycin | PMID:28851864 | In the mNG2(1-10) fragment, mutations are K128M, S142T, R150M, G172V and K213M; in the mNG2(11) fragment, mutation is V15M. | Backbone Size:5296; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | split before mutations | 2026-08-15 01:20:59 | 1 |
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pEGFP_sfCherry2(11)_Keratin Resource Report Resource Website |
RRID:Addgene_82604 | sfCherry2(11)_Keratin | Homo sapiens | Kanamycin | PMID:28851864 | Backbone Marker:Michael Davidson Fluorescent Protein Collection at the UCSF Nikon Imaging center; Backbone Size:5000; Vector Backbone:pEGFP-mEmerald-Keratin14-N-18; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | changed Gly12 to Ala in sfCherry11+ | 2026-08-15 01:20:59 | 0 | |
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pCAG-scFvGCN4sfGFPTET1CD Resource Report Resource Website 10+ mentions |
RRID:Addgene_82561 | scFvGCN4sfGFPTET1CD | Kanamycin | PMID:27571369 | Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:57 | 14 |
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