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Species: Homo sapiens
Genetic Insert: CenpC
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Comments: This CenpC sequence is codon optimized for expression in insect cells.
Proper citation: RRID:Addgene_171490 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32694211
Proper citation: RRID:Addgene_171604 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: Mdn1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4858; Vector Backbone:pFastBacHT C; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32694211
Proper citation: RRID:Addgene_171603 Copy
Species: Synthetic
Genetic Insert: C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
Vector Backbone Description: Vector Backbone:PINII; Vector Types:Multisite Gateway entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34211131
Comments: Multisite Gateway entry vector to express one hand of pTALECD-UGI. Use Platinum Gate TALEN Kit (Kit # 1000000043 ) for assembly of TALE repeats, and use this plasmid in last step.
Proper citation: RRID:Addgene_171724 Copy
Genetic Insert: GCaMP7b
Vector Backbone Description: Vector Backbone:pAAV1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34146468
Proper citation: RRID:Addgene_171689 Copy
Genetic Insert: PCnU6-sgRNA_scaffold
Vector Backbone Description: Backbone Size:4866; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791226
Proper citation: RRID:Addgene_171686 Copy
Genetic Insert: Zeocin
Vector Backbone Description: Vector Backbone:pSBbi; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33979199
Proper citation: RRID:Addgene_171685 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32694211
Proper citation: RRID:Addgene_171605 Copy
Genetic Insert: Neomycin
Vector Backbone Description: Vector Backbone:pSBbi; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33979199
Proper citation: RRID:Addgene_171684 Copy
Vector Backbone Description: Backbone Size:4835; Vector Backbone:pSBbi; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33979199
Proper citation: RRID:Addgene_171681 Copy
Genetic Insert: SARS-CoV-2 Nucleocapsid
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171710 Copy
Species: Bacillus thuringiensis
Genetic Insert: Glycosyltransferase ThuS
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5293; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34283964
Proper citation: RRID:Addgene_171674 Copy
Species: Homo sapiens
Genetic Insert: Ratiometric pHLuorin2 fused to B4GALT1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pRpHluorin-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171719 Copy
Species: Synthetic
Genetic Insert: Ratiometric pHLuorin2 targeted to ER
Vector Backbone Description: Backbone Size:4730; Vector Backbone:pRpHLuorin2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171717 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171716 Copy
Species: Bacillus subtilis (strain 168)
Genetic Insert: Glycosyltransferase SunS
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5293; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34283964
Proper citation: RRID:Addgene_171673 Copy
Genetic Insert: Cas12i1 D647A
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:5972; Vector Backbone:p2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32519675
Proper citation: RRID:Addgene_171671 Copy
Species: Synthetic
Genetic Insert: TRE-NpHR-EYFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36509775
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.10.13.464095v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171621 Copy
Species: Xenopus laevis
Genetic Insert: goosecoid promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8543150
Comments: Generated by PCR using the 3' primer (nucleotides - 5 to
+ 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into
pOLuc.
Proper citation: RRID:Addgene_17158 Copy
Species: Xenopus laevis
Genetic Insert: goosecoid promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8543150
Comments: The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987).
Proper citation: RRID:Addgene_17159 Copy
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